Higher trigger spike counts matched 64–69% of one-way electrical calls in two mouse cortical maps

We tested cell factors in two optical electrical maps of mouse layer 1. The planned test used separate pairs detected in both directions. It predicted one-way call direction in 59.1% of 1,287 pairs and 55.4% of 1,175 pairs. A post hoc rule chose the direction with more usable trigger spikes. This count rule gave 63.8% and 69.1% correct directions. Central 95% cell resample ranges were 58.1–69.4% and 64.1–74.5%. Cells passed source quality checks and were 60–400 micrometres apart. Adding cell factors after count control gave uncertain log loss decreases of 2.60% and 1.54%. The association supports a sampling balance check before these calls set direction in a circuit model. It does not establish equal junction conductance or show the effects of equal sampling. Count analyses were added after the first results. The source is DANDI 001960, version 0.261013.2016. The package preserves code, source arrays, predictions, 999 cell resamples per mouse, figures, plans and amendments. Fresh remote extraction matched all 42 arrays. A complete repeat matched all 17 analysis files. These two maps do not test an animal population or provide an anatomical gap-junction census.

electrical coupling · optical electrophysiology · detection · trigger count · reciprocity · mouse cortex · neural circuits