Ozone slopes were uncertain after collection-month control in six exposed-anther herbarium samples from 1941 to 2017
We tested the link between total-column ozone and floral UV pigmentation in 934 exposed-anther herbarium specimens. Six tests covered Australia, Eurasia and North America in 1941-1985 and 1986-2017. Models controlled species-specific collection months and time trends, monthly temperature and location. All six family-wise 95% intervals included zero. Ozone slopes ranged from -4.14 to +1.41 percentage points of UV-absorbing petal area per +10 Dobson units. Month controls removed 64-91% of the exposure variation left by the other controls. Equal-species weights and a transformed response gave the same slope signs, but did not give clear effects. These tests use disjoint specimens from one archive, not new independent archives. Few species, nonrandom collection, source exposure estimates and unmodeled shared errors limit inference. The results do not establish an ozone effect or its absence. Total-column ozone differs from surface ozone pollution. The package includes code, a specimen panel, source hashes, selection records, numerical checks and retrieval instructions.
herbarium · biodiversity · total column ozone · temperature · collection season · observation bias · floral pigmentation · regional replication · null result
References
6- doi:10.5061/dryad.ttdz08kw1
- doi:10.1016/j.cub.2020.08.077
- doi:10.1016/j.cub.2021.06.019
- doi:10.1016/j.cub.2021.06.020
- doi:10.1016/j.cub.2021.05.022
- useshttps://zenodo.org/records/4002484
Version 1 workbook and source analysis code; data license CC0.
Cited by
10 publicationsPublic catalog entries on Sciscape.
- reviewsSpatial cell controls gave no clear change in floral pigment trends in six herbarium samples from 1941 to 2017
Prior ozone and month-control tests motivate the geographic-cell extension. No record tables were reused.
- usesSix herbarium samples did not establish a common temperature slope difference between anther groups in 1941-2017
Reuses its raw source dataset and broad region-period design; tests a new temperature contrast with new code.
- usesSix herbarium samples gave uncertain temperature slopes for adjusted pigment spread in 1941-2017
Uses its public source choice and broad region-period split. Specimens overlap; code and spread estimand are new.
- usesSix herbarium samples did not show a clear ozone and temperature interaction in floral UV pigment in 1941-2017
Its same-archive results and region-period design inform this new interaction test. No code or specimen table was reused.
- usesLocal collection controls removed 65-87% of residual ozone variation in four Eurasian and North American herbarium samples
Uses its verified source description, sample definitions and baseline model. New code reproduces its baseline and adds local geographic controls. Specimens overlap.
- reviewsMonth control gave no clear change in floral pigment trends in six herbarium samples from 1941 to 2017
Prior conditional ozone test in the same source archive motivates the comparison of exposure support with time support.
- usesTemperature curvature was uncertain in six adjusted exposed-anther herbarium samples from 1943 to 2017
Builds on its region-period split and collection controls. Source records overlap; no independent archive validation.
- reviewsCollector-label controls retained less than 15 percent of time variation in four adjusted herbarium samples from 1941 to 2017
Prior seasonal or spatial support audit motivates a new collector-label design audit on overlapping source records.
- usesExact species-year controls retained less than 30 percent of separate climate information in six floral pigment samples from 1941 to 2017
Uses its sample rules, period split and comparison model. Biological records fully overlap; new code tests exact species-year control.
- usesTwo or three specimens supplied half of adjusted ozone information in three herbarium samples from 1941 to 2017
Use its sample split and model for a new support and bounded-error audit. Raw specimens overlap.