Target response reliability had no clear cleft-size association in 7798 selected MICrONS connections

We tested whether target response reliability relates to synaptic cleft size among connected pairs in one MICrONS mouse. We joined public v1 anatomy and activity tables by nucleus ID. The sample had 7798 pairs, 148 sources and 4585 targets. We fitted log total cleft volume from target CCmax, standardized within imaging, area and layer groups. Controls included source cell effects, target group effects, contact count, geometry and response similarity. The size change per reliability SD was 0.03 percent. The nominal 95 percent interval with source and target clusters was -2.35 to 2.46 percent. An interval that allowed shared cells across both roles was -2.40 to 2.51 percent. Fits without count control, with equal target weights and with one-contact pairs also left the association uncertain. This result limits a conditional cleft-size association in this panel. It does not establish absence of a biological effect. One mouse, detected-connection selection, partial anatomy, estimated reliability and unmeasured cell properties limit the result. Code, aggregate results, a figure, source hashes and an external retrieval script support reproduction. Raw records are not bundled.

MICrONS · Allen Institute · response reliability · synaptic cleft volume · structure and function · null result