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With normalized count weights, keeping unlisted inputs reduced cone route majorities from 265 to 6 in 341 HFseg1 midget cells

We tested whether a dominant bipolar input establishes a dominant cone route in one draft human retinal graph. We used HFseg1 table version 20250805 and 341 selected midget ganglion cells. We independently reproduced an earlier SCI result: 290 cells had one same-polarity midget bipolar source with more than half of all incoming counts. We then traced labelled cone inputs through these sources. Edge weights were counts normalized at each target. A cone route coefficient above one-half gives a sufficient contrast-sign guarantee in a bounded two-stage linear model. With only labelled cone and bipolar inputs in the totals, 265 cells passed, with 150 distinct cone IDs. Retaining unlisted source counts at both stages reduced these counts to 6 cells and 6 cones. Including every recorded input gave zero passing cells. An exploratory stage test gave 44 passing cells when unlisted inputs were kept only at bipolar cells, and 229 when they were kept only at ganglion cells. In the model that omitted unlisted inputs, 147 distinct cones still had a guaranteed majority route when the maximum edge efficacy was twice the minimum at each target. Exact rational arithmetic checked all 3,192 nominal results. Full corner tests checked the efficacy bound. A new run with fresh source downloads reproduced all numerical outputs and the headline figure. These results locate a source-coverage limit on a count-based cone-channel claim. They do not show loss of single-cone receptive fields. Limits include one draft sample, unreviewed cells, assumed count weights, input normalization, and omitted feedback, electrical coupling, and measured noise. The package includes code, source hashes, result tables, proofs, figures, and repeat instructions.

human retina · fovea · midget ganglion cells · cone signals · connectome coverage · synaptic counts · linear models · robust bounds

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References

4
  1. usesdoi:10.1073/pnas.2603286123

    We analyze the public HFseg1 chemical graph with table paths 20250805_20250805. We preserve exact source URLs, object version IDs and hashes. Original files remain external.

  2. One midget bipolar source had over half of the input counts in 290 of 341 selected ganglion cells in the HFseg1 draft human retina graph sci:sha256:f05bf9643a9d02ad9ab8e6ec193ea3b941a26f94e0b5b74e6cacf9a63c2ebed2

    [Legacy relation: reproduces] We independently reproduce its 341-cell cohort and 290 bipolar count majorities from the original source tables. We extend the analysis to upstream cone routes and source coverage. We reuse no code or derived data.

  3. doi:10.1016/j.neuron.2020.09.014

    This study links selective human foveal midget circuits to single-cone resolution and motivates a test of the complete cone-to-output path.

  4. doi:10.1016/j.visres.2025.108751

    This review describes differences between anatomical and physiological midget receptive fields. It motivates the stated limits on our count-model claim.

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