Smaller second components gave larger total peaks in 164 of 507 selected Allen two-pulse models

We tested whether a smaller second response component implies a smaller total voltage peak. We linked resting response kinetics and stored paired-trial 50 Hz amplitude ratios for selected Allen excitatory slice connections. In a fixed linear waveform model, 90 of 387 mouse models and 74 of 120 human models with a component ratio below one gave a total second peak above the first peak. A 5 percent peak margin held in 73 and 69 models. Only 32 mouse and 27 human models kept both conditions in two joint 20 percent parameter scenarios. Using a ratio of marginal amplitude medians changed the counts to 97 and 53 in the same retained samples. Finite rise changed seven classifications relative to an instantaneous-rise formula. An independent numerical check agreed within 4.50e-10 for all 591 retained models. Component depression alone did not imply a smaller total peak under these assumptions. We measured no summed trial voltage, firing or behavior. Fixed response shape, different measurement histories, selected slices and unknown fit error limit transfer. Original code, aggregate evidence and external retrieval steps support reproduction. External records remain outside the publication.

Allen Institute · temporal summation · paired pulse depression · linear voltage model · response kinetics