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q-bio.NC · Neurons and Cognitionstat.ME · Methodology

With at least three locations per connection, 1 micrometre depth omissions gave excess loss over source position controls in eight C. elegans maps

We tested direct connection loss after a local omission in eight fixed C. elegans brain maps. The panel had 162 embryonic neuron labels. We merged records at the same source skeleton location. The main test used connections with at least three locations and a uniformly placed depth slab of width 1 micrometre. Mean loss probabilities in two adult maps were 0.545% and 0.411%. Exact source position control means were 0.115% and 0.0819%, giving ratios of 4.76 and 5.01. The controls kept source positions, connection counts and complete target groups fixed. Keeping target counts within 4 micrometre depth bins reduced the ratios to 2.60 and 2.51. All eight main scores exceeded their source control ranges, with Holm-adjusted Monte Carlo p values of 0.004 to 0.005. More locations still protected many connections. In the adults, 71.5% and 82.7% of tested connections had zero loss probability under this slab model. Width, axis, source-weight and record-definition checks kept the direction of the effect. Exact finite calculations, exhaustive small cases and a full repeat passed. The package gives original code, source versions and hashes, all scores, figures and repeat instructions. The reference does not keep pair-specific membrane contacts fixed. This is a sensitivity test of direct anatomical support. It does not establish actual reconstruction error, neural damage or a change in function.

connectome · Caenorhabditis elegans · synapse locations · spatial omissions · connection reliability · exact probability · conditional controls

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References

4
  1. usesdoi:10.1038/s41586-021-03778-8

    Source measurements for the eight synapse lists and skeleton maps, pinned at repository commit 0646af9d25896ae660f97d462eab2d67282f5625.

  2. usesWith all recorded chemical edges among 162 embryonic neurons, longer paths bypassed most two-step site bottlenecks in eight C. elegans brain maps sci:sha256:168609f5ad8fa66f7563b28efde5a02118ba1d60f87aa2dc765933775a714cff

    We use its CC BY 4.0 list of 162 embryonic brain neuron names. We extend the shared-site loss question to a defined spatial omission model. We do not reuse its cut algorithm.

  3. doi:10.1073/pnas.2201699120

    Prior evidence for synapse clustering along worm neurites. The present contribution is an exact matched omission benchmark, not the first report of clustering.

  4. doi:10.1038/s41586-021-03284-x

    Prior evidence that local membrane contacts constrain wiring. Our source position controls do not keep pair-specific membrane contact geometry fixed.

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