Hook axon calcium rose more after DNg100 activation than in adjacent control ramps in five restrained flies

We tested whether the calcium rise after DNg100 activation exceeds the rise during nearby control ramps of the leg. We used released hook axon traces from five restrained Drosophila flies. Each fly had five activation trials between six control trials. The main change was the mean signal at 1.25 to 1.75 seconds minus the mean at 0.25 to 0.75 seconds after activation ended. We subtracted the mean change in both adjacent controls. All five fly means were positive. Their equal-fly mean was 0.862 delta F/F. A control-only movement model left 0.860 delta F/F. Both main exact two-sided sign tests gave p = 0.0625. The measured mean had a t interval of 0.379 to 1.344 delta F/F under independent normal animal effects. The mean separate GFP detector-control change was -0.00025 delta F/F in three flies. Previous-only controls, following-only controls, trial medians, and two other window pairs kept positive mean contrasts. An audit of the released MANC graph found positive DNg100 input to three left chief 9A cells and negative output from them to 29 hook cells at a five-synapse cutoff. All 74 circuit counts matched an independent export. The source study already reported the calcium rise. The new result is its persistence with adjacent controls, delayed windows, and a movement model tested on excluded control trials. Imaging was absent during activation. This result does not measure inhibition during activation or prove that 9A cells mediate the rise. The package preserves code, exact source data, hashes, complete fits, predictions, figures, checks, and repeat steps.

Drosophila · proprioception · presynaptic inhibition · DNg100 · 9A neurons · connectome · calcium imaging · motor command