In one fly study, late to early calcium ratios rose in three of nine controlled 1 mM dopamine tests after separate baseline subtraction

We tested a common output gain rule for dopamine in fly olfactory glomeruli. We used released paired calcium traces for DL1, DL5 and DM3, three odors, 1 mM dopamine, and external solution controls. Each group had 6 to 13 paired records. We subtracted a separate pre-response baseline from each trace. A timing audit corrected the first planned baseline, which included part of the 3-octanol response. The final late to early ratio change after solution adjustment was 0.276 for DL1 with 3-octanol, 0.235 for DL1 with MCH, and 0.131 for DM3 with 3-octanol. These three of nine label tests passed a Holm 0.05 threshold. The tests assumed exchangeable treatment groups. The same three point effects remained with a second safe baseline. One DL5 ratio was unstable because its early response was small. In a separate test, one gain fitted to two odors reduced error on the excluded odor by 34.6% in DL1 and 18.2% in DM3, but increased it by 77.4% in DL5, averaged over three fixed odors. The model received observed solution drift. These results constrain a common gain for mean calcium traces. They do not identify a synaptic mechanism or reject a fixed anatomical network. The source study had already shown odor-dependent modulation. Our contribution is a baseline audit, a defined shape test, an odor exclusion benchmark, and reusable code and statistical summaries. Calcium signals, a high applied dose, missing global animal identifiers, and one source study limit inference.

Drosophila · dopamine · antennal lobe · calcium imaging · response timing · gain model · baseline subtraction · connectome calibration