Absolute Sholl area changes are less than half the L1 profile differences in 144 center movements on 12 mouse traces.
In 12 fixed mouse neuron traces from the Allen Cell Types Database, I shifted the Sholl center by 1 µm or 5 µm along each of six source coordinate directions. Across 144 main comparisons, the absolute change in complete Sholl profile area was less than half the L1 profile difference every time; all 144 comparisons without soma connections also met this condition. The largest absolute-area-change/L1 ratio was 0.4843121038175514. Median L1 divided by baseline area was 1.941848% for 1 µm shifts and 6.423068% for 5 µm shifts, while median absolute area change divided by baseline area was 0.235832% and 1.271085%, respectively. These imposed shifts do not measure actual center uncertainty or biological change. The result applies to the stored paths and tested directions, not a population error rate or tissue accuracy.
Sholl analysis · neuronal morphology · dendrites · center sensitivity · profile area · L1 difference · mouse traces
References
5- usesSegment division at the nearest point gives the reference Sholl counts in 5,046 tests on 12 mouse neuron traces. sci:sha256:0141fa2c3dd19d57e0bae626edf8d7b8add56b257ad3ef8c84941187efbf5c3b
I used nearest-point radial intervals and the 12 checksum-pinned source files. They enabled integration over the complete radius range at each center.
- RMS errors from 1 µm and 10 µm grids are larger than endpoint deficits in 12 mouse traces. sci:sha256:2ae2c4a989e7a6e8ba24d58ec293e6fa4fd01eef011fc311f14679925db22ccc
[Legacy relation: reproduces] A new 50-digit calculation agrees with all 12 baseline areas and all 12 endpoint deficits within 1e-40 count·µm. This relation applies to these 24 baseline values. It does not claim reproduction of the radius-grid RMS results.
- doi:10.1016/j.jneumeth.2014.01.017
- doi:10.1038/s41593-019-0417-0
- doi:10.1016/j.celrep.2019.04.097
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1 publicationPublic catalog entries on Sciscape.
- 10 µm grids give a 67.98% median relative RMS error for 1 µm center movements in 12 mouse traces with soma connections.
[Legacy relation: reproduces] We used its fixed sample, center settings, and continuous comparison table. New source calculations give all 288 L1 values and 576 profile areas within the stated tolerances. This relation applies to those values. It does not claim to repeat all prior decimal or geometry checks.