Three known release settings isolate a shared release covariance term in a linear model with fixed source statistics, gains and recording noise
One synaptic site can serve several target neurons. Shared source activity can hide the covariance from shared release. We derived a test for a direct linear input model. If a known common factor scales release probabilities, target covariance is quadratic in this factor. Three settings isolate its linear coefficient. Separate contact release has zero off-diagonal linear coefficients. Source statistics, gains and recording noise must stay fixed. We used 28,344 site incidences from eight C. elegans brain maps to rank candidate pairs. SMBDR and SMBVR shared 18 and 12 sites in the two adults. For this pair, a design with a low middle release scale detected shared release in 85.8% and 87.2% of simulated experiments. A reference design detected it in 12.7% and 21.1%. Each design used 2,400 trials and each setting had 1,000 simulated experiments. Exact fourth moments explain the precision cost. A changing source correlation can produce the full false signal while source marginal moments, linear target means and extra polynomial checks stay unchanged. Thus the method needs calibrated release scales and independent source controls. The study provides proofs, data, pair rankings, code, figures and repeat instructions. It does not measure shared release in worms.
connectome · polyadic synapse · shared release · covariance · experiment design · C. elegans · source activity · release probability
References
5- usesIndependent connection models overstate two-route reliability in eight C. elegans brains under independent loss of synapse sites sci:sha256:e4e968f83ac4898fed7ad12dae72e6e8007240a0bd95c1d5ea014ad90cd9fd48
We reuse its attributed site incidence table and neuron scope. We check them against the pinned source files.
- With unit weights and free source covariance, two linear input models gave equal covariance on an 81-neuron panel in two adult C. elegans maps sci:sha256:473ffba1f697779e4c002af488e06f8041c313f5f444977db0549f2cc6615780
This study showed that free source covariance can hide a shared site covariance difference. We test an intervention model.
- usesdoi:10.1038/s41586-021-03778-8
The eight brain reconstructions supply the original site and neuron annotations.
- doi:10.1002/dneu.20307
The study recorded concurrent miniature currents in neighboring worm muscle cells. It supports the biological question.
- doi:10.1016/S0006-3495(01)75848-1
This prior work used means, variances and covariances to estimate synaptic parameters. It provides method context.
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