In three released mouse data sets, local rank correlations of SV2A fluorescence were 0.14 with PSD95 and 0.15 with gephyrin

We tested whether SV2A mean fluorescence varies with nearby postsynaptic marker fluorescence. A recorded plan used 81 paired image tiles from three released mouse data sets. An added follow-up used all 271 common numbered gray matter tiles for SV2A with PSD95, gephyrin or synaptophysin. We retained mutual nearest points at most five pixels apart. We ranked matched intensities and removed mean rank within 256 pixel blocks. The full follow-up gave equal weight to available regions within mice and to mice. Mean local correlations were 0.143 for PSD95 and 0.149 for gephyrin. The planned sample gave 0.136 and 0.149. Full valid image counts were 59 of 85 and 87 of 97. The available synaptophysin images gave 0.469, with 45 of 89 images valid. Only one region had a valid synaptophysin estimate in Mouse 2. A later control for partner brightness 16–64 pixels away gave adjusted correlations of 0.133, 0.102 and 0.416 in a different retained sample. Brightness filters removed most local gephyrin samples. Position shifts showed that chance proximity can contribute to the selected pairs. These are small positive postsynaptic associations in released detections. They do not establish synaptic strength or protein counts. The TIFF headers and paper give different image dimensions, so distances remain in pixels. The package preserves all used coordinate files, source records, code, figures, sensitivity tests and offline repeat instructions.

SV2A · PSD95 · gephyrin · synaptophysin · synaptic proteins · fluorescence · spatial controls · point matching · reproducible analysis