In 9 mice with one calcium indicator, Pvalb, Sst, Vip and Lamp5 neurons in V1 did not keep grating tuning more stable across days than IT excitatory neurons

Question: are mouse V1 interneurons more stable across days than excitatory neurons? A prior campaign study (sci 5d0863e3) found a large Pvalb advantage in Allen Visual Coding 2P (+0.139 in the stability metric S), but there only Pvalb mice had the slower GCaMP6s indicator. Data: DANDI:002015 (Allen Institute, draft): 9 Snap25-Cre x Oi4 (jGCaMP8s) mice, 8 planes, 3 identical drifting-grating sessions per mouse (0.8-28 days apart), Xenium ABC-atlas labels of the same neurons. Method: S = between-session tuning correlation (82 grating conditions) divided by the early-late correlation inside each session; fixed effects for mouse x plane; controls for reliability, amplitude, days and running-state change; two-stage (mouse, neuron) bootstrap. Results (13,518 neurons): differences against L2/3 IT were Pvalb +0.024 (95% CI -0.016 to +0.066), Sst -0.001, Vip -0.009, Lamp5 +0.030 (all CIs include 0); L4/5 IT -0.001. Pvalb had 13% less instability (CI -9% to +36%) against 69% in the prior study; at reliability >= 0.5 its difference was +0.002, as also in the prior study (+0.025, n.s.). IT supertypes added under 0.1% of variance. Population coupling gave +0.003 S per SD (CI includes 0). S fell 0.026 per doubling of days. Sst and Vip changed response amplitude less than IT. One session (sub-786297, 2025-05-14) has stimulus times offset by about 1 s; we excluded it (correction gave the same results). Limitations: few interneurons (85-310 per subclass), gratings are near ceiling stability, registration errors decrease group differences, running state changed between sessions in many mice.

representational drift · mouse V1 · two-photon imaging · spatial transcriptomics · Xenium · Pvalb · Sst · Vip · interneurons · tuning stability · population coupling · DANDI 002015 · replication · data quality