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q-bio.NC · Neurons and Cognition

At 5 micrometres, relay scores gave no clear gain in a fixed ridge test of calcium responses from 101 C. elegans worms

We tested whether distances between input and output synapses inside relay neurons help predict calcium responses after stimulation. We used two adult C. elegans maps and 9,070 event-target observations from 101 other worms. The final test had 7,519 animal-direction means and 2,708 directions. Each local score sums exponential distance weights through 153 connected relay skeletons. The main distance scale was 5 micrometres. An exact input-label reference preserves relay counts, input positions, and target output positions. The test held out complete animals. Controls included chemical and gap paths, cell classes, soma distance, source responses, and assay terms. Adding observed local scores increased mean squared prediction error by 0.056%. The 95% range from 300 animal refits was a 0.387% error increase to a 0.266% decrease. Thus, the main test gave no clear gain. Alternative scales, maps, animal splits, red-signal correction, and image geometry gave small changes. Added artificial local signals could be detected. Across 7,211 anatomical directions, adjusted local scores had rank correlation 0.628 between the two fixed maps. This anatomical agreement does not establish a local physiological mechanism. The result applies to the selected calcium measure and fixed models. It does not exclude local voltage, release, or calcium-store effects. The package has source data, score matrices, code, source hashes, fitted states, checks, figures, and offline repeat instructions.

connectome · C. elegans · local signal transfer · neurite · synapse position · calcium response · signal propagation · reproducible analysis

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References

13
  1. usesAnatomical paths reduced mean calcium prediction error in 103 C. elegans animals excluded from training sci:sha256:a10115ec5ae0e7b414c52276d1b6d39d2529e9168b00f3fed9ac6719192f7206

    We use its verified event-labelled response facts, reference chemical and gap matrices, and raw data provenance.

  2. usesSoma distance predicted detected connections in the C. elegans signal propagation atlas more than EM wiring, and near responses followed in-plane image distance within neuron pairs sci:sha256:30698592c6fd42752f488ca2d57b112feb1cdb3fc7b5a7501d42f83a9a48da7a

    We use its verified soma atlas facts and recording image geometry for prediction controls.

  3. Synapse spacing had correlation 0.239 in two adult C. elegans maps after count, cell-class and geometry summary controls sci:sha256:79c699c56a0060f4e53b7a7357956b174b9e43241f49232ef32ea403365f1466

    This study tested adult agreement of presynaptic spacing. Our study tests local input-output routes inside relay cells.

  4. Synapse spacing gave no clear gain in a fixed ridge test of 271 calcium response means from 65 C. elegans worms sci:sha256:eee6853c5962440436ecf0b945979c8f27bccdd4846732bfffe87046295e37ee

    This prior response test used within-connection presynaptic spacing. The present score instead measures separation through intervening neurons.

  5. usesdoi:10.5281/zenodo.17846991

    This CC BY 4.0 fixed archive supplies the exact adult skeleton and chemical synapse files.

  6. usesdoi:10.1038/s41586-021-03778-8

    Witvliet and colleagues made the adult electron microscopy observations.

  7. usesdoi:10.1186/s12915-026-02539-1

    Balshayi and colleagues prepared and archived the anatomical source files used here.

  8. usesdoi:10.1038/s41586-023-06683-4

    Randi and colleagues made the signal propagation observations summarized by the response benchmark.

  9. usesdoi:10.48324/dandi.001075/0.240930.1859

    This fixed physiological release is the raw source of the reused response and image geometry facts.

  10. usesdoi:10.1038/s41586-019-1352-7

    The reused benchmark supplies canonical neuron names and reference chemical and gap graph facts from Cook and colleagues.

  11. usesdoi:10.1186/s12859-022-04738-3

    Skuhersky and colleagues supply the soma atlas used for Euclidean distance controls.

  12. doi:10.1073/pnas.2201699120

    Ruach and colleagues proposed local functions for input-output synapse groups within neurites.

  13. doi:10.1038/nature11081

    Hendricks and colleagues measured local calcium-store signals in RIA. This result motivates the question and limits a passive voltage interpretation.

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