At gain 0.8, predicted transmitter signs gave higher wing coactivity scores than all 199 shuffles within cell class and side in two linear fly models
We tested whether inhibitory relays improve a fixed link between fly wiring and wing muscle calcium coactivity. We used all 18,926,219 connection rows in female BANC v888 and male MANC v1.2.1 simple tables. A linear model included signed paths of all lengths at fixed gain 0.8. The functional panel had 55 pairs of 11 muscles from 377 selected movies. Direct scores were 0.084 and 0.097. Signed path scores were 0.102 and 0.124, against 0.041 and 0.031 for matched unsigned paths. Each supplied-sign score exceeded all 199 sign shuffles that kept sign counts by source cell class and side. The upper reference tail was 0.005 in each map. An added reference also fixed input and output strength bins; its tails were 0.005 and 0.030. However, full muscle-label tails for the signed scores were 0.222 and 0.182. Overall agreement was weak. A one-muscle omission check also weakened the stricter MANC result. The findings show that sign placement adds limited information in this model. They do not show a close or causal account of muscle coactivity. The package preserves code, exact small data and annotations, plans, figures, results, reference draws, source hashes, and repeat instructions.
connectome · Drosophila · motor control · wing muscles · inhibition · transmitter signs · linear model · BANC · MANC · structure and function
References
10- usesShared input in three fly maps had correlations of 0.08 to 0.11 with calcium coactivity of 11 wing muscles in selected female flight bouts sci:sha256:66ad0d92d95cfafcb44299b13e579f1d10dd8d43a336f4c4216914befc0abf1f
We reuse its verified muscle trace extracts, quality masks, target matches, and extraction rules. We extend its direct and unsigned path tests with signed paths of all lengths and conditional sign references.
- doi:10.1038/s41586-024-07600-z
This study links common premotor input to motor groups and muscle function.
- usesdoi:10.1038/s41586-024-07293-4
This study supplies the wing muscle calcium recordings and source quality rules.
- usesdoi:10.22002/aypcy-ck464
We use CaltechDATA version 1.0 and preserve its exact HDF5 source hash.
- usesdoi:10.7910/DVN/7WTH1N
We use dataset version 3.0, BANC v888 metadata, and the compiled v3 simple edge table.
- usesdoi:10.1038/s41586-026-10735-w
The BANC reconstruction supplies the female brain and nerve cord map.
- usesdoi:10.7554/eLife.97769.1
The MANC reconstruction supplies the male nerve cord map, version 1.2.1.
- usesdoi:10.7554/eLife.97766.1
The MANC annotation work supplies cell class and transmitter annotations through the compiled metadata.
- doi:10.1016/j.cell.2024.03.016
Predicted transmitter identity can inform a model sign, with uncertainty about each synaptic effect.
- doi:10.1038/s41586-024-07763-9
This signed connectome model provides context for structure-to-function tests. We do not reproduce its spiking model.
Cited by
3 publicationsPublic catalog entries on Sciscape.
- usesAt fixed target scales and gain 0.8, source variances bounded by one million fitted 11 wing muscle correlations in two signed fly connectome models
Supplies the complete signed graph model, graph cache, source labels, motor targets and MIT extraction code. We test bounded unequal source variance.
- supportsWiring similarity of fly wing steering motor neurons in four connectomes matched flight co-activation of basalar and first-axillary muscles (Spearman 0.65) but not of the other six muscles (-0.12)
Our 564 signed whole-nerve-cord model variants with source-specific noise also gave weak fits that did not exceed the muscle-label null (best 0.31, max-statistic p = 0.46).
- In three fly nerve cord connectomes, EM transmitter predictions broke the hemilineage rule in the same early-born neurons, and secondary neurons kept the rule
Uses predicted transmitter signs of BANC v888 and MANC nerve cord neurons in signed models; our sign comparison applies to such models.