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q-bio.NC · Neurons and Cognitionq-bio.QM · Quantitative Methods

Cav2.1–Munc13-1 pairs within 50 nm were below random label means in five cerebellar map groups from two mice

We extended an established marker separation finding with a checked graph benchmark and local controls. We used 111 paired Cav2.1 and Munc13-1 maps from two mice and held each map's marker counts and positions fixed. The main sample had 26 granule cell maps and 20 stellate cell maps from one mouse. At 50 nm, the mean observed-to-reference ratios were 0.567 and 0.392, respectively. Both lower-tail tests had Holm-adjusted p=0.00010 with 19,999 random label assignments. Three protocol groups from a second mouse gave ratios of 0.625, 0.645, and 0.561. Later controls that kept marker counts within 100 nm grid cells gave ratios of 0.747–0.781 in the main maps. Merging nearby same-type labels also reduced the deficit. These results show structured marker labels in fixed recorded maps. They do not identify molecular repulsion, calcium sensor distance, or release probability. The package gives an applied graph tool, exact reference moments, source files, figures, checks, all results, and instructions to repeat the work.

synapse structure · calcium channel · Munc13-1 · Cav2.1 · cerebellum · random label test · molecular maps · synaptic release

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References

7
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    This pinned source gives all molecular coordinate files and source protocol records.

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    We reuse its verified paired-coordinate arrangement, source subset, and animal labels. We independently rebuild all coordinates and test a new marker mixing reference.

  3. Uniform samples of six labels reduced 40 nm pair clustering calls to 54% in 102 mouse stellate cell profiles with conservative ties sci:sha256:35cc1247cd0ee868f687cc62fd04da8029fd2942317c3908812e45bdeea12c37

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    The source study links molecular topography to synaptic release through separate experiments and models.

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    [Legacy relation: reproduces] The 2018 source preprint already used random marker labels in a bivariate Ripley analysis of 26 GC maps. Our fixed-radius score repeats its qualitative departure from random mixing. We add a graph benchmark and local controls.

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    This work explains the difference between random label and spatial independence tests. That distinction limits our biological interpretation.

  7. doi:10.1016/j.neuron.2021.08.037

    This is the source article erratum. We checked its record but did not obtain its full text. Our numerical input is pinned to dataset version 3.

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