Measured visual response correlation exceeded null means with overlap weights and fixed cell totals in one MICrONS V1 panel

Do synapses link cells with similar visual responses after both cell count totals are fixed? We tested one MICrONS mouse V1 panel with 3,208 synapses, 36 source axons and 91,647 candidate pairs. Each candidate had positive axon-dendrite overlap length. The observed mean measured movie response correlation per synapse was 0.09444. A conditional Poisson allocation law with overlap weights and the same source and target synapse totals gave a mean of 0.06968 and a central 95% allocation range of 0.06461 to 0.07502. All eight planned weight and layer checks retained a positive excess. The primary law generated too many distinct connected pairs. We therefore added later tests that fixed all 283 multiple-synapse pairs and moved only 2,557 single-synapse links. These tests also kept every original cell synapse total, partner degree and multiplicity count fixed. With source scan totals and a distance penalty together, the observed single-synapse score was 0.08167 versus a null mean of 0.07589, with a range of 0.07258 to 0.07917. Five of 32,000 saved states exceeded the observed score. These later tests are exploratory. The result supports pair-specific measured response matching under the stated allocation laws. It does not establish a wiring mechanism, causal transmission or transfer to other mice. We supply a sampler with long cycle moves for forbidden pairs, exact small-table checks, extreme-start checks, all compact inputs and results, figures, source hashes and repeat instructions. Markov chain samples are correlated, and allocation ranges are not uncertainty intervals across animals.

connectome · structure and function · MICrONS · visual cortex · synapse allocation · fixed margins · visual response matching · conditional sampling