Two adult C. elegans chemical maps gave no clear gain over two early maps in a fixed test of 8,265 calcium response directions

We tested whether adult chemical wiring predicts adult calcium response detection better than early larval wiring. We used all eight Witvliet brain maps. The main comparison used two early and two adult maps, with all contacts among 162 embryonic brain neurons. We joined the graphs to the Randi wild-type signal propagation atlas. The test had 8,265 directed pairs and 600 detections at q < 0.05. Each pair had at least three finite stored response values. Fixed logistic models used chemical counts, normalized walks, input similarity, measurement count, soma distance, adult electrical counts, and neuron-class terms. Five folds excluded all fitting labels that involved a test source class. Adult Brier error was 0.056311, against 0.056633 for early maps. The reduction was 0.000322, or 0.57 percent. A descriptive central 95 percent class-weight range was -0.000456 to 0.001583. After adult synapse records were sampled to match early source counts, the reduction was 0.000082. Common controls had lower error than either chemical model, at 0.055928. A check with all 180 brain neurons gave a similar small difference. The evidence gives no clear adult-map prediction gain in these models. It does not show equal function across ages. Functional data came from adults only, and structural and functional animals differed. The package preserves code, source hashes, transformed inputs, models, predictions, sampling records, figures, and repeat instructions. All input hashes and 127 numerical output files passed a fresh-source repeat.

connectome · Caenorhabditis elegans · development · signal propagation · structure and function · optogenetics · prediction · synapse sampling