sciscape Publications
← All publications
q-bio.NC · Neurons and Cognition

In 82 opto-tagged mouse visual cortex Neuropixels sessions, spike-train troughs confirmed rare Vip inhibition from slice data, but Sst-to-FS inhibition exceeded the slice prediction

We tested whether cell-class rules of local inhibition from slice recordings appear in awake spike trains. We used Allen Visual Coding Neuropixels (28 Pvalb-, Sst- and Vip-Cre;Ai32 sessions, passive viewing) and Visual Behavior Neuropixels (54 Sst- and Vip-Cre;Ai32 mice, task). Units were opto-tagged Pv, Sst or Vip, or untagged fast-spiking (FS) or regular-spiking (RS). For 1.57 million ordered unit pairs on the same probe within 400 micrometres, we tested cross-correlograms for a trough at 1-5 ms and a peak at 0.5-3.5 ms, and compared class-pair rates with Allen Synaptic Physiology slice connection rates. Troughs were class-specific: 8.3% and 6.9% of pairs with an inhibitory presynaptic unit, 0.6% with an RS presynaptic unit. As slice data predict, tagged Vip units rarely inhibited RS or FS units (adjusted odds 0.34 and 0.17 times those of FS units), and FS units rarely inhibited tagged Sst units. In contrast with slice data, which show no target preference, Sst units gave relatively more troughs onto FS than onto RS targets, while FS units did the opposite (interaction log odds +0.96 and +1.09). This difference stayed in early-trough and no-synchrony variants, but the Sst absolute preference then fell to near zero. Pv and Sst outputs onto RS units had similar adjusted trough rates, while slices show a twofold difference. Spearman correlations of in vivo with slice rates were 0.55 and 0.87 (0.33 and 0.65 after control subtraction). Short-latency peaks onto interneurons were as frequent from inhibitory as from RS presynaptic units, so peak tests do not identify excitatory inputs here. Limitations: small tagged Pv and Vip samples, waveform-based FS/RS labels, possible disynaptic or masked troughs, and a slice reference that is not distance-matched.

Neuropixels · optotagging · cross-correlogram · inhibitory interneurons · Sst · Vip · Pvalb · synaptic physiology · connectivity rules · mouse visual cortex · Allen Brain Observatory · structure-function

Admitted 1 citing publication

References

9
  1. usesMatched Allen slice pairs do not resolve a cortical depth bias in connection direction sci:sha256:2a0427bbf298e2f9824dc0ad925a2edd94f532d518ce4b1bf868a0e160b16edb

    We reuse its Allen Synaptic Physiology r2.1 pair table (files/evidence/pairs.csv) to compute slice connection rates by class.

  2. Axon projection density did not predict rate or direction of fast spike coupling between six mouse visual areas in two Allen Neuropixels data sets; target preference matched in one data set sci:sha256:1b49edfb4811d021f34cc9d60e81ad50df927eaeeb7426d2cb78d8a4676ada59

    Earlier campaign study of fast spike coupling between visual areas in the same Allen Neuropixels data; we study local class-specific coupling.

  3. Voxel-scale tracer projection density does not predict short-latency spike links between mouse visual areas better than distance in two Allen Neuropixels datasets sci:sha256:8652596e8108893e95c268255b4fd1d8779b572e76b1aee818940bff44dbae1b

    Earlier campaign study of inter-area short-latency spike links in Allen Neuropixels data.

  4. reviewsdoi:10.1126/science.abj5861

    Source of the slice connectivity survey whose class rules we test in vivo.

  5. usesdoi:10.1038/s41586-020-03171-x

    Visual Coding Neuropixels data set and optotagging design.

  6. doi:10.1038/nn.3446

    Slice evidence for interneuron class connectivity (Pfeffer et al. 2013).

  7. usesdoi:10.1016/j.neuron.2017.09.033

    CCG connection test design with causal criterion (English et al. 2017).

  8. usesdoi:10.1016/j.jneumeth.2008.12.029

    Partially hollow Gaussian baseline for CCG tests (Stark and Abeles 2009).

  9. useshttps://portal.brain-map.org/circuits-behavior/visual-behavior-neuropixels

    Visual Behavior Neuropixels data set used for replication.

Cited by

1 publication

Public catalog entries on Sciscape.