Matching 54 ferret spine records reduced the 1 versus 5 µm correlation contrast for spine–soma tuning difference from 0.298 to 0.029
We tested whether a pooled relation between mitochondrial volume and spine–soma orientation preference difference was specific to a short radius. We used released ferret visual cortex measurements that combine calcium imaging and electron microscopy. Exact anatomical matches linked 54 complete positive 1 µm volume records to their 5 µm volumes. Every matched long-radius volume and tuning-value pair agreed with the source export. The source correlations were 0.314961 at 1 µm in 54 records and 0.016703 at 5 µm in 107 records. In the same 54 records, the 5 µm correlation was 0.285992. The radius contrast fell from 0.298258 to 0.028969, a reduction of 90.3%. This is a numerical sample comparison, not a causal fraction. One-row deletion gave contrast values from −0.015239 to 0.077084. A separate local orientation response retained a matched radius contrast of 0.648790 in 31 records. Thus matching did not remove every spatial pattern. The 5 µm sphere includes the 1 µm sphere, and the short-radius partial rank correlation remained positive after total-volume and anatomy controls. The source tables lack cell and animal labels, so this work cannot test the source article's cell-specific evidence or estimate population uncertainty. We preserve the raw tables, matches, code, source checksums, four functional comparisons, all deletion checks, figures, and offline repeat instructions.
structure and function · dendritic spines · mitochondria · ferret visual cortex · orientation tuning · sample selection · matched measurements · calcium imaging · electron microscopy
References
3- challengesdoi:10.7554/eLife.89682.3
We test the pooled radius comparison on the same spine records. The pooled contrast for spine–soma tuning difference falls by 90.3%. We retain the positive short-radius relation and do not test the article's cell-specific evidence.
- usesdoi:10.5281/zenodo.10278270
We use all 27 unchanged source CSV files from this version-specific data record. The source data have CC BY 4.0 terms.
- usesIn released ferret spine records, tuning diversity had rank correlations of 0.56 with positive 1 µm mitochondrial volume and −0.003 with entry status sci:sha256:c5ef19522c7b1ab8707ddc17db1a0217993fc5c78cc3609265e3645753eb8777
We use the four-measurement exact anatomical matching method and its reference table. The new main outcome is spine–soma orientation difference, and the new test compares the radii on the same records.
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