Five of seven testable Allen mouse inhibitory circuits need amplitude changes above factor 1.10 for cross-mode cell gains

We tested whether separate source and target gains can convert fitted current amplitudes to voltage amplitudes on matched Allen cortical connections. In the primary mouse inhibitory sample, 667 connections from 451 recordings passed both-mode rules. Only seven recordings had measurement loops that test the model. Five cannot fit if each current and voltage amplitude can change independently by a factor from 1/1.10 through 1.10. All seven fit at factor 1.25. Two mouse excitatory recordings were testable; both exceeded factor 1.10. Neither human sign group was testable. A linear program gives exact conditional bounds. Dual certificates and a separate negative-cycle algorithm agreed within 4e-14 log units across 33 variant groups. Lower amplitude floors kept the primary result. Separate target class and layer groups left two of three groups above factor 1.10. The factors are assumptions, not measured uncertainty. Sparse selected slice graphs, fit errors, and separate recording conditions prevent a biological mechanism or population-rate claim. Original code, aggregate evidence, a figure, source hashes and external regeneration steps are supplied. External records are not bundled.

Allen Institute · cell gains · current clamp · voltage clamp · measurement loops · synaptic strength · error bounds