With input and output layouts fixed, a mean distance test retained no corrected call in 72 reciprocal pairs from one adult C. elegans map

We tested whether opposite chemical synapse directions occupy separate regions beyond each neuron's general input and output layout. The sample had 72 reciprocal pairs from one compiled adult C. elegans head map. We used a contrast of mean path distances. A comparison that exchanged direction labels retained three pairs after Holm correction. A reference that kept all neuron input and output positions fixed retained one pair in its initial 99,999 draws. We made one fixed numerical refinement with 4,999,999 new draws per endpoint. For the strongest pair, RMDDL–RMDVR, the adjusted tail estimate changed from 0.04536 to 0.05440. Its 95 percent numerical range was 0.05243 to 0.05641. No pair then retained a call under this mean distance test. Fixed domains within 0.5 and 1 micrometre of partner skeletons also retained no pair, among 63 and 72 eligible pairs. A rank check on the three known pairs kept a positive point result for the strongest pair. Thus, the result depends on the reference and statistic. It does not show equal distributions or absent local computation. We provide the exact reference-mean identity, source hashes, extraction code, all test results, random seeds, numerical bounds, figures, and repeat instructions. Original source data remain external. The test assumes exchangeable partner membership within fixed direction pools. It does not preserve every anatomical contact or measure local neural function.

connectome · Caenorhabditis elegans · synapse position · neurite · local computation · input and output layout · conditional randomization · energy statistic