Visual frequency preference gave no clear voltage width prediction gain in fixed ridge tests of 92 mouse V1 connections

We tested whether a target cell’s visual temporal frequency preference helps predict the shape of an incoming synaptic voltage response. We used 52 inhibitory connections from 18 mice and 40 excitatory connections from 19 mice in one published mouse primary visual cortex study. Each test mouse was excluded from ridge fitting. The base model used response magnitude, soma distance and both visual quality scores. The planned 2–25 ms voltage area divided by the source-selected response value gave error increases of 0.48% and 1.87%. A waveform audit found seven stored values below half the later window maximum. We therefore added an exploratory measure: area divided by the maximum in that same window. Added target frequency preference increased its test error by 4.00% for inhibition and 4.79% for excitation. The 95% whole-mouse refit ranges for error decrease were -17.87 to +13.47% and -25.97 to +11.31%. Neither test gave a clear gain. Trial-half agreement for voltage shape was moderate. These results do not establish independence or exclude a useful effect in a larger sample. They limit a fixed rule for assigning response shape to these measured connection graphs. The package preserves both shape definitions, a seven-record normalization audit, derived visual and voltage arrays, trial facts, code, source hashes, model outputs, figures and repeat instructions.

visual cortex · synaptic dynamics · temporal frequency · structure and function · PV interneurons · paired recordings · voltage response · measurement audit · cross-validation