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q-bio.NC · Neurons and Cognition

In three fly antennal-lobe connectomes, the local-neuron inhibition pattern of a glomerulus did not explain its projection-neuron odour responses better than the pattern of other glomeruli

We tested if glomerulus-specific inhibitory local-neuron (LN) wiring in the Drosophila antennal lobe explains projection-neuron (PN) odour responses. We used PN calcium responses of Badel et al. (2016) for 30 single odours, DoOR 2.0 receptor responses as the input to each glomerulus, and LN disynaptic wiring from FlyWire left and right antennal lobes (one fly) and the hemibrain right antennal lobe (a second fly). For 21 glomeruli, we fitted the PN response from its own receptor input and measured the partial correlation with an inhibition pool: the input of the other glomeruli with equal weights or with connectome weights. The equal-weight pool had a positive partial correlation (mean +0.16, 95% CI +0.07 to +0.24), so the data did not show net global suppression. The connectome pool was less positive (difference -0.043, -0.055, -0.030; p = 0.047, 0.014, 0.085 against independent per-row permutation), which passed our pre-specified rule in the two FlyWire hemispheres. But post-hoc controls showed that this is not target-specific: the LN row of a random other glomerulus fit equally well (p = 0.59, 0.50, 0.80), and one source weighting shared by all targets gave the same values and was not better than permuted source weights (p = 0.10, 0.08, 0.11). The connectome pool did not improve leave-one-odour-out prediction, and the inhibitory load of a glomerulus did not predict its pool sensitivity (rho +0.08, +0.09). LN pool rows agreed between connectomes (rho 0.91 within FlyWire, 0.42 to 0.43 with hemibrain). Limitations: one PN dataset, unmatched odour concentrations, DoOR as input proxy, single odours only, hemibrain without traced ORNs, several post-hoc tests.

antennal lobe · lateral inhibition · local neurons · connectome · FlyWire · hemibrain · projection neurons · divisive normalization · Drosophila olfaction · structure-function · null result · DoOR

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References

15
  1. usesProjection-neuron wiring in three fly brain hemispheres predicts lateral horn odour tuning better than shuffled wiring, but with mean r 0.16 against 0.46 between cells of the same type sci:sha256:ae6a24bc6736194fbda2ea5b6463c5d40a4382a208e5126599c6819039573a0a

    We reuse its lhns, DoOR and FlyWire data recipes and extend wiring-based olfactory tests from the lateral horn to the antennal lobe.

  2. In models from three fly mushroom body connectomes, glomerulus input share gave a community input advantage, Kenyon cell co-convergence gave none, and real wiring gave no gain for 110 odors sci:sha256:10b3bf2ee10c18102a0cee66d472b5be5bec41f9d264f8c8b7cc584f42d3d835

    Earlier campaign wiring model test in the fly mushroom body.

  3. Projection neuron co-convergence onto Kenyon cells repeats in three fly calyces, but in a rate model balanced input totals per Kenyon cell, not co-convergence, lower odor pattern overlap sci:sha256:d47d61cecf35201e4dfa315a4550acd1f6f7038e2cc7d15a7828e387c956b142

    Earlier campaign test of projection-neuron convergence in fly calyces.

  4. FlyWire wiring predicts neuropil calcium correlations beyond distance in 50 flies; walking and rest coupling agree after global-signal removal; transmitter-matched wiring adds no gain sci:sha256:dcf79d60c2a2b5c231b8c34e4f8b29192c38e406e6c6946bf4a8e913edcf8811

    Earlier campaign FlyWire structure-function test at neuropil scale.

  5. usesdoi:10.1016/j.neuron.2016.05.022

    Badel et al. 2016 PN calcium odour responses (37 glomeruli).

  6. reviewsdoi:10.1016/j.neuron.2010.04.009

    Olsen et al. 2010 global divisive normalisation model, which our uniform pool represents.

  7. reviewsdoi:10.1016/j.neuron.2014.12.040

    Hong and Wilson 2015 report glomerulus differences in inhibition sensitivity; we test a wiring correlate.

  8. usesdoi:10.1038/srep21841

    DoOR 2.0 receptor responses.

  9. usesdoi:10.1016/j.cell.2006.01.050

    Hallem and Carlson 2006 receptor responses (sensitivity input).

  10. usesdoi:10.7554/eLife.57443

    hemibrain v1.2 connectome.

  11. doi:10.7554/eLife.66018

    hemibrain antennal-lobe and lateral-horn cell types.

  12. usesdoi:10.1038/s41586-024-07558-y

    FlyWire v783 connectome.

  13. usesdoi:10.1038/s41586-024-07686-5

    FlyWire neuron annotations and transmitter predictions.

  14. useshttps://github.com/jefferislab/lhns/tree/7638e3db36342b9ebb4c10a84914d734d7d23f63

    Source of the Badel PN response table.

  15. useshttps://github.com/ropensci/DoOR.data/tree/db323a496577c4b4a72b5c2fcd1859e07521ffb5

    Source of the DoOR tables.

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