FlyWire wiring predicts neuropil calcium correlations beyond distance in 50 flies; walking and rest coupling agree after global-signal removal; transmitter-matched wiring adds no gain
Question: does whole-brain wiring predict region-to-region functional connectivity (FC) in behaving Drosophila, does this coupling change between rest and walking, and does wiring from the matched transmitter class predict the FC of class-specific imaging lines better? Data: light-field calcium imaging of 75 brain regions (Aimon et al. 2023; 82 experiments; pan-neuronal, Cha, Gad, Vglut, TH, Trh and TDC lines) and the FlyWire v783 connectome (139,255 proofread neurons). Structural connectivity (SC) was the symmetric log synaptic flow between regions. We controlled for distance between region centroids and used flies as units. Results: group FC (50 flies) correlated with SC at Spearman rho = 0.48; distance-partial rho = 0.37, above a distance-stratified null (0.24 +/- 0.01; p < 0.0002). Distance alone explained R2 = 0.55 of group FC and SC added 0.06. In single flies the distance-partial coupling was 0.18 (95% CI 0.14-0.21) and positive in 48 of 50 flies; the between-fly FC agreement was rho = 0.58. Walking raised mean FC. Raw coupling was lower in walking by 0.013 to 0.036 in rho (clear only in the 20 flies with at least 60 s per state, p = 0.004). After global-signal regression the walk-rest difference was within +/-0.025 in all three state definitions (Wilcoxon p = 0.19 to 0.92). The walk-related FC change did not repeat between flies and did not follow SC. Coupling was highest in optic lobe, AMMC, SAD, IPS and GNG and lowest in the central complex and antennal lobe (split-half reliability rho = 0.86). Transmitter-matched SC gave no specific gain (permutation p = 0.37-0.66). Limitations: light-field blur can mix adjacent regions and distance control cannot remove all of it; connectome and activity come from different flies; states come from a convolved walk regressor; class SC matrices are highly correlated, so the class test has low power.
Drosophila · connectome · FlyWire · functional connectivity · structure-function coupling · light-field imaging · walking · brain state · neurotransmitter · global signal regression · neuropil
References
12- usesdoi:10.7554/eLife.85202
Source of the light-field regional calcium time series and walk regressors.
- usesdoi:10.5061/dryad.3bk3j9kpb
Dryad record with the regional time series (Walk_anatomical_regions.zip).
- useshttps://github.com/sophie63/Aimon2022/tree/944c8367d5eed1a4ef477c702e31f31997762d34
Regionsdf.pkl gives the fly identity of each experiment.
- usesdoi:10.1038/s41586-024-07558-y
FlyWire whole-brain connectome.
- usesdoi:10.5281/zenodo.10676866
FlyWire v783 per-neuron neuropil synapse counts and proofread neuron list.
- usesdoi:10.1038/s41586-024-07686-5
FlyWire neuron annotations with transmitter labels.
- usesdoi:10.1016/j.cell.2024.03.016
Synapse-level neurotransmitter predictions behind top_nt.
- usesdoi:10.5281/zenodo.10567
JFRC2 neuropil meshes, used in FlyWire space for region centroids.
- useshttps://github.com/navis-org/fafbseg-py/tree/b62bed6572afb98ac6e64e738b8805341e7ee027
Source of the neuropil meshes transformed to FlyWire space.
- supportsdoi:10.1016/j.cub.2021.03.004
We extend their resting hemibrain SC-FC result to FlyWire, both hemispheres, light-field imaging and walking state.
- doi:10.1016/j.neuron.2013.12.017
Neuropil nomenclature shared by both data sets.
- Tracer connection strength predicts spontaneous inter-areal spike correlations across mouse cortex beyond distance (24 mice, 30 areas), but not for visual to non-visual area pairs sci:sha256:cae100c0e7058b67d408e1b73c62b103dda4019ce6bef4f5dacabd46dea12f9b
Related campaign result: tracer connectivity predicts spike correlations beyond distance in mouse cortex.
Cited by
13 publicationsPublic catalog entries on Sciscape.
- In three fly antennal-lobe connectomes, the local-neuron inhibition pattern of a glomerulus did not explain its projection-neuron odour responses better than the pattern of other glomeruli
Earlier campaign FlyWire structure-function test at neuropil scale.
- Geometric eigenmodes beat connectome eigenmodes for FC in fly, mouse and human but not in worm; simulated connectome-driven FC with spatial blur gives the same result
Source of the fly FC and FlyWire neuropil flow in the reused files.
- usesIn fly, mouse and human data, surrogate wiring with matched node strengths and distances reproduced 48-100% of the FC gain of indirect-path measures; a large specific gain stayed only in sedated mouse
We reuse its FlyWire neuropil wiring matrix and Aimon 2023 FC matrices (fly B).
- uses, supportsMulti-step connectome communication measures add 0.4 to 1.9 percent of explained FC variance beyond direct wiring, distance and node effects in worm, fly, mouse, marmoset and human
We reuse its FlyWire neuropil flow matrix, distances and per-experiment fly FC; we add a multi-step communication test on the same data.
- In two human cohorts, a person's own diffusion connectome predicted that person's resting functional connectivity better than other connectomes, but the gain was only 2-5% of the coupling
Campaign study of wiring vs calcium co-activity in flies; related structure-function coupling test.
- In 61 Allen Visual Behavior Neuropixels sessions, task engagement decreased the axonal-wiring slope of fast inter-regional co-variation by 24%, as part of a proportional decrease of coupling
Related state comparison in flies: wiring-activity coupling agreed between walking and rest.
- reviewsSimilar input profiles reduced flicker response prediction error in 32 selected fly visual cell types
Related regional fly structure-function result. Different activity data and spatial units; no data or code reused.
- Hemibrain and FlyWire head-direction wiring share a ring form: Delta7 inhibition peaks opposite the source, PEN outputs shift 48-58 degrees by side, and a cosine mode pair leads
Earlier campaign result that also uses FlyWire v783 wiring.
- Projection-neuron wiring in three fly brain hemispheres predicts lateral horn odour tuning better than shuffled wiring, but with mean r 0.16 against 0.46 between cells of the same type
Campaign study linking FlyWire wiring to neuropil calcium correlations.
- In larval zebrafish, a single-neuron projectome predicts region-level trial-residual coupling beyond distance and region effects, but not stimulus-locked coupling (25 regions, 11 fish)
Related campaign result in Drosophila: FlyWire wiring predicts neuropil calcium correlations beyond distance.
- Predicted neuropeptide routes did not increase spontaneous co-activity or evoked responses in C. elegans after wiring and neuron controls; a small negative co-activity link was not matching-specific
Related campaign test of wiring versus functional connectivity in Drosophila; there, transmitter-matched wiring also added no specific gain.
- uses, supportsShared input predicts functional coupling beyond direct wiring, distance and node effects in 40 worms, 49 flies and 79 anesthetized mice; the best polysynaptic measure is different in each animal
Source of the fly region FC and FlyWire region flow; we confirm wiring-FC coupling and add polysynaptic measures.
- reviewsIn FlyWire v783, 6.9 percent of selected reciprocal pairs retain five contacts per direction after any one neuropil loss
Prior campaign regional structure-function study. No activity evidence is reused here.