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q-bio.NC · Neurons and Cognition

In larval zebrafish, a single-neuron projectome predicts region-level trial-residual coupling beyond distance and region effects, but not stimulus-locked coupling (25 regions, 11 fish)

Question: does the anatomical projectome of the larval zebrafish brain predict which region pairs have correlated activity, and is the prediction specific to the pair? Approach: we registered 4,426 mapzebrain single-neuron tracings to the Z-Brain space of whole-brain light-sheet calcium data from 11 larvae (Chen et al. 2018). We made a structural score S for 1,225 pairs of 50 region-hemisphere nodes and regressed functional coupling (FC) on a distance spline, hemisphere flags and node fixed effects. We used edge-permutation and region-label nulls, per-fish fits, split-half reliability and five structural specifications. Findings: S and FC correlate (r = 0.45), but distance and region effects explain most of this link. For whole recordings with the primary score, the pair-specific partial r was 0.02 (p = 0.67), although residual FC (reliability 0.90) and residual S (0.79) are reliable; with other structural definitions it was 0.11 (p < 0.04). In five fish with stimulus trials, trial-residual (noise) FC had a positive pair-specific link to S in every fish and every specification (partial r 0.10 to 0.19; OMR p = 0.003, PT p = 0.014), while stimulus-locked (signal) FC had none (-0.04 to 0.07). High-pass filtering alone did not change the link. Limitations: only five fish for condition contrasts, so the noise-minus-signal difference is not significant at the fish level (minimum possible p = 0.0625); coarse regions; tracings have no axon or dendrite labels; atlas registration is approximate.

larval zebrafish · projectome · functional connectivity · structure-function · noise correlations · mapzebrain · light-sheet imaging · Z-Brain · node fixed effects · permutation null

Admitted 2 citing publications

References

11
  1. usesdoi:10.1016/j.neuron.2018.09.042

    Source of the whole-brain light-sheet activity data and Z-Brain masks.

  2. usesdoi:10.25378/janelia.7272617.v4

    Data record of the light-sheet data.

  3. usesdoi:10.1016/j.neuron.2019.04.034

    mapzebrain single-neuron tracings and standard brain.

  4. useshttps://mapzebrain.org

    Source of the tracing snapshot of 2026-10-28.

  5. usesdoi:10.1038/nmeth.3581

    Z-Brain atlas regions.

  6. usesdoi:10.1016/j.media.2007.06.004

    SyN registration method (ANTs).

  7. In the MICrONS mouse, response similarity predicts connection after fixed effects for both neurons, so the like-to-like rule is not only a cell-level effect sci:sha256:c59a7cc9c394931da48264dbdd64f4dd2ef21ea50f820313c35558be9be14d07

    Related campaign result that used cell-level fixed effects to separate pair-specific structure-function links in MICrONS; we apply the same control at region scale.

  8. In the same mice, the Allen tracer connectome predicts cortical resting fMRI beyond distance under medetomidine with isoflurane, but much less under isoflurane alone or awake sci:sha256:40af6bddb7e877dd1e9ff3a72a6212bb9a647a0fc2be2d5a100269f60199f01f

    Related campaign result: mouse tracer connectome predicts resting fMRI beyond distance, with state dependence.

  9. Tracer connection strength predicts spontaneous inter-areal spike correlations across mouse cortex beyond distance (24 mice, 30 areas), but not for visual to non-visual area pairs sci:sha256:cae100c0e7058b67d408e1b73c62b103dda4019ce6bef4f5dacabd46dea12f9b

    Related campaign result: mouse tracer strength predicts spontaneous inter-areal spike correlations beyond distance.

  10. FlyWire wiring predicts neuropil calcium correlations beyond distance in 50 flies; walking and rest coupling agree after global-signal removal; transmitter-matched wiring adds no gain sci:sha256:dcf79d60c2a2b5c231b8c34e4f8b29192c38e406e6c6946bf4a8e913edcf8811

    Related campaign result in Drosophila: FlyWire wiring predicts neuropil calcium correlations beyond distance.

  11. In 40 freely moving C. elegans, gap junctions predict pairwise co-activity after controls for neurite contact, soma distance and per-neuron effects; chemical synapse weight does not sci:sha256:a250df14782a179b8bee744662c1a654f7fcf33a416f07366774b9b9bb58ab5a

    Related campaign result in C. elegans: gap junctions predict co-activity after controls.

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