Calcium adjustment retained a positive mitochondrial volume and tuning diversity relation in 23 ferret spine records

We test whether measured local calcium activity accounts for a relation between nearby mitochondrial volume and local orientation diversity. We use public ferret visual cortex tables that link calcium imaging and electron microscopy. Twenty-three positive-volume records have all required measurements. On these same records, the rank correlation is 0.518 before adjustment and 0.562 after adjustment for local calcium amplitude and temporal correlation. Adjustment also for total mitochondrial volume within 5 micrometres and soma distance gives 0.539. This value stays positive after every deletion of one row or two rows; the two-row range is 0.257 to 0.746. Thus these measured calcium summaries do not remove the relation under linear rank adjustment. The result describes one released table. Missing cell, animal, and neighborhood labels prevent population inference or tests of transfer. Selection, measurement error, and unmeasured factors can still explain the relation. We provide matched records, source checks, code, and a figure.

structure and function · mitochondria · dendritic spines · ferret visual cortex · calcium imaging · orientation diversity · rank adjustment