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q-bio.NC · Neurons and Cognitionstat.ME · Methodology

Sampling rules changed the sign of the bilateral weight overlap difference in 150 neurons from four L1 C. elegans brains

We tested how contact counts affect bilateral wiring comparisons in the eight Witvliet et al. (2021) brains. We fixed 150 embryonic neurons in 75 left/right pairs. The primary difference was mean weight overlap at 8 and 16 hours minus mean overlap at 0 and 5 hours. The planned primary edge overlap difference changed from +2.03 points in full graphs to -4.38 points after count matching. The secondary weight overlap difference in full graphs was +2.82 percentage points. Uniform samples of 1,003 contacts without replacement gave -5.72 points, with a central 95% sampling range of -8.28 to -3.19. Sampling 520 whole synaptic sites gave -6.27 points. A control copied the first graph four times without changing its relative weights. Count matching then reduced overlap from 64.41% to 48.72%. Thus sampling alone can cause a decline. Equal contact draws with replacement gave an L1 difference of +0.60 points, with a sampling range of -3.29 to +4.29. The sign of this graph comparison depends on the sampling rule. These ranges describe sampling from fixed graphs, not variation among animals. The data do not establish a population change in symmetry. We supply exact expected mirror edge counts, verified source checks, code, result tables, figures, and reproduction instructions.

Caenorhabditis elegans · connectome · bilateral symmetry · development · synapse count · sampling · rarefaction · polyadic synapses · reproducible analysis

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References

5
  1. usesdoi:10.1038/s41586-021-03778-8

    We use the eight chemical synapse datasets, neuron metadata, ages, and published asymmetry definition. We pin the source repository to commit 0646af9d25896ae660f97d462eab2d67282f5625.

  2. doi:10.1016/j.isci.2024.110713

    This study also examines developmental bilateral symmetry, with a different graph definition.

  3. doi:10.1371/journal.pone.0013701

    This study explains how graph size and density affect comparisons of brain networks.

  4. doi:10.7554/eLife.83739

    This study shows that conclusions about bilateral symmetry depend on the graph model and definition.

  5. usesdoi:10.2307/1934145

    We use standard finite population absence probabilities and extend them to expected mirror-paired edge counts, including shared sites.

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