With linear time control, prior stimuli to the same neuron had a negative fluorescence response slope in 67 C. elegans records

We tested whether earlier stimuli to one neuron mark a lower response after recording time is controlled. We read the fixed DANDI 001075 release 0.240930.1859. The panel had 456 events in 206 source groups from 67 C. elegans records. Each group had equal recorded light settings. The model used group means and a separate linear time slope per record. The common slope was -0.439 transformed fluorescence units per prior stimulus. Its 95% whole-record bootstrap range was -0.791 to -0.087. Green-only, longer-window, and quadratic-time checks also had negative slopes with ranges below zero. Time control left separate count information in 47 records. The effective count was 28.6 records. The mean of separate record slopes had a wide range across zero. A secondary direct-wiring target contrast also had a range across zero. The source result applies to a common slope and the tested time models. It does not identify neural adaptation, optical effects, or synaptic change. The package gives source code, event facts, exclusion reasons, source hashes, all resamples, figures, and repeat steps.

connectome · signal propagation · source calibration · stimulation history · calcium imaging · Caenorhabditis elegans