Mean source fluorescence responses fell across repeated stimuli with equal recorded light settings in 66 C. elegans records

Do equal recorded light settings give stable responses during repeated neuron stimulation? We matched the first and last retained stimulus for each source neuron in a record. The data came from DANDI 001075 release 0.240930.1859. The main panel had 205 groups of repeated stimuli in 66 C. elegans records. Each group had the same measured targets at both times. No response-size screen selected the panel. The response was the green fluorescence change after an asinh transform, minus the same red-channel transform. Each record had equal weight. Mean source response fell by 0.956 transformed units. The 95% record bootstrap range was -1.203 to -0.715. The green-only fractional response fell from 0.340 to 0.185. For 176 groups in 61 records, the contrast between directly connected and unconnected targets fell by 0.113 units. Its range was -0.213 to -0.010. The target result was less stable under selection checks and a matched before-stimulus check. The source decrease persisted in green alone and after the before-stimulus check. These results measure chronological response changes in selected fluorescence records. They do not separate repeated stimulation from elapsed time, or source activation from indicator and imaging effects. The package gives matched data, raw-source checks, code, figures, and repeat instructions. The new benchmark tests an input-stability assumption used in structure-to-function studies.

connectome · signal propagation · C. elegans · optogenetics · calcium imaging · response stability · repeated stimulation