Direct wiring weights had uncertain terms in a linear test of repeated calcium responses from 46 C. elegans records

We tested whether direct wiring marks a stronger relation between source and target calcium changes across repeated activation of the same source. We used 5,080 target observations from 243 stimuli in 46 C. elegans records. A linear model removed each record/source/target mean and local time trends. It controlled source pre-response and source and target red changes. The outcome compared transformed target post and pre changes. For a contrast from zero to three chemical EM sections, the added source-response slope was +0.027. Its central 97.5% range from 1,999 record bootstrap refits was -0.125 to +0.203. For zero to two electrical sections, the slope contrast was +0.070, with range -0.201 to +0.233. All six planned checks had ranges across zero. Time removal left chemical predictor variation in 25 records and electrical predictor variation in 18. We prove why two repeats from one source can lose all source-response information under a local linear time control. The results do not establish an added direct-count association or its absence. The source readout is a network outcome, counts have no signs, and the reference anatomy came from other animals. The package preserves code, checked data, source hashes, all fits, figures and repeat instructions.

connectome · Caenorhabditis elegans · signal propagation · repeated stimulation · source response · within-pair changes · time controls · electrical synapses