Electrical counts had a positive adjusted term for calcium response correlation in 23 C. elegans records, but repeat coverage changed the estimate
We tested whether electrical wiring marks shared calcium response changes across repeated single-neuron stimulation in C. elegans. We checked 48,300 inherited observations against 103 original NWB files and added protocol and red-channel controls. We removed each target's mean within each repeated source group. The main panel contained 4,314 animal and target-pair rows from 23 animals, with 100 target neurons and 96 distinct electrically linked pairs. A regression controlled animal, neuron identity, chemical wiring, pre-stimulus correlation and red-channel correlation. Each animal had equal weight. The electrical-count coefficient was 0.1022 per unit of log(1 + count), with a 95% range of 0.0126 to 0.2220 from 2,000 complete animal bootstrap refits. The fitted contrast from zero to two electrical sections was +0.112 correlation units. Broader and stricter repeat-coverage rules each gave about +0.046, with ranges that included zero. Higher label confidence and source-response controls also gave uncertain estimates. Removing any one animal or neuron kept the main estimate positive. Added coverage terms left it almost unchanged. The result supports a positive conditional association in the main selected panel, with limits from sparse repeats and panel selection. It does not identify electrical conductance, intrinsic noise, or a change caused by stimulation alone. The package preserves code, checked event data, pair summaries, all fits, bootstrap draws, source hashes, figures and reproduction instructions. A clean repeat reproduced the source extraction and all fitted results exactly.
C. elegans · electrical synapses · calcium imaging · optogenetics · repeated stimulation · response covariance · connectome · sampling sensitivity
References
10- usesWiring features reduced calcium response error by 1.17% beyond cell identity in a fixed test across 103 C. elegans worms sci:sha256:0e5e26303629eecdd516b6e9419e39fd70425547e36bb717a69612e587e68861
We reuse its exact animal-labelled event table and Cook graph, verify its signed source package, and check all response values against the original NWB files.
- usesdoi:10.48324/dandi.001075/0.240930.1859
The fixed processed NWB release supplies the original signals, stimulus protocol values and new red-channel controls.
- usesdoi:10.1038/s41586-023-06683-4
This source study collected the neural stimulation and calcium measurements.
- usesdoi:10.1038/s41586-019-1352-7
The corrected July 2020 hermaphrodite workbook supplies the chemical and electrical reference graphs.
- doi:10.1073/pnas.2022599118
Experiments linked electrical synapses to coordinated activity in a defined C. elegans neuron pair.
- doi:10.1038/ncomms5442
Artificial electrical connections changed neural response properties and behavior in C. elegans.
- doi:10.1101/2024.09.22.614271
This preprint fitted a connectome-constrained dynamical model to neural perturbation data.
- doi:10.1371/journal.pcbi.1014152
Simulations linked electrical wiring to functional communities and coordinated model responses.
- Electrical links marked positive calcium coactivity after behavior projection in 21 C. elegans baseline recordings sci:sha256:44c4743246c563f7c4caa0330db5b1cd3dbdf22edfa5286f97fe758fbf793b67
Prior SCI work tested electrical wiring and calcium coactivity after behavior controls in freely moving worms.
- With behavior and wiring controls, electrical links had a larger calcium correlation term at 0.01–0.05 than 0.05–0.20 Hz in 21 C. elegans recordings sci:sha256:3c071fa4e6e3971dc3c4e0a67030d77eb2f7df00ca0af5b266b75919be61289e
Prior SCI work tested the frequency dependence of electrical-wiring terms for spontaneous calcium coactivity.
Cited by
5 publicationsPublic catalog entries on Sciscape.
- usesDirect wiring weights had uncertain terms in a linear test of repeated calcium responses from 46 C. elegans records
We reuse its verified augmented event table and anatomical graph. We adapt its MIT channel-alignment and response-window code. Our test relates source and target changes within a repeated direction.
- Electrical neighbor means gave no clear added prediction gain in a ridge test across 92 C. elegans worms
This prior study tested electrical wiring and shared responses across repeated stimuli.
- usesWith 8 s windows, C. elegans pairs selected for fluorescence decreases had positive mean changes relative to the earlier trend in 11 animal splits
Its event keys and graph arrangement are inherited through the matched-window package.
- Electrical counts had no clear adjusted link to response change agreement in 87 C. elegans recordings
This close prior study tested repeat-centered calcium response correlation and reported selection sensitivity. We extend the question with a different score and broader event panel.
- usesMatched windows in 23 C. elegans worms showed no clear increase after light in the electrical count term for response correlation
We reuse the verified event keys, anatomical graph, source receipts, earlier pair table and MIT signal extraction code. We test its stated limit: its positive adjusted coefficient did not establish a rise above baseline.