Electrical links marked positive calcium coactivity after behavior projection in 21 C. elegans baseline recordings

We tested whether electrical anatomy marks shared calcium activity after adjustment for recorded behavior. We used the full available Cook hermaphrodite connectome with 302 neuron slots and named neurons from the Atanas and Kim data. The main panel had 54,645 observations of 8,168 somatic neuron pairs in 21 baseline recordings. Pairs of the same neuron class were excluded. A separate regression in each recording controlled chemical wiring, shared inputs and outputs, soma distance, ganglion, and endpoint neuron identity. Within-record behavior projection reduced the mean electrical-link coefficient from 0.098 to 0.044 Fisher z units, a 55% reduction. The residual mean had a 95% recording bootstrap interval of 0.033 to 0.055 and was positive in 20 of 21 recordings. In 19 other recordings before a heat stimulus, the mean was 0.036, with an interval of 0.024 to 0.050. The association remained with measured membrane contact controls on restricted panels. Independently shifted neural traces gave mean coefficients near zero. A planned behavior model failed prediction diagnostics. We preserve that run and identify the revised analysis as exploratory. The result is conditional on fixed anatomy and the fitted behavior basis. It does not estimate direct electrical conductance. The package includes figures, derived pair data, source hashes, code, and repeat instructions. A second complete run reproduced seven main output files byte for byte.

C. elegans · connectome · gap junction · calcium imaging · behavior adjustment · shared neural activity · membrane contact · reproducibility