With behavior and wiring controls, electrical links had a larger calcium correlation term at 0.01–0.05 than 0.05–0.20 Hz in 21 C. elegans recordings

We tested whether the relation between electrical wiring and calcium coactivity depends on frequency. We used 21 baseline recordings of freely moving C. elegans and all 302 neuronal slots in the corrected Cook map. The analysis kept all 3,671 chemical edges and 1,091 electrical edges. It used 75,602 animal-pair entries. Each animal model controlled endpoint identity, measured behavior, chemical wiring, neuron class and atlas distance. The mean electrical term was 0.0914 Fisher z units at 0.01–0.05 Hz and 0.0504 at 0.05–0.20 Hz. The mean paired difference was 0.0410, with a 95% animal bootstrap interval of 0.0323 to 0.0492. All 21 animal differences were positive. The contrast remained positive after exclusion of same-class pairs and with either of two adult maps. A sensitivity range from varying animal and class weights was wider, at 0.0040 to 0.0991. The result describes a frequency-specific structure–function association. Calcium filtering, noise and shared drive can affect this relation. It does not identify direct electrical conductance. The package includes band summaries, full graph facts, source checksums, code, figures and repeat instructions.

C. elegans · connectome · electrical synapses · calcium imaging · frequency · structure and function · gap junctions · behavior controls