Electrical links in three C. elegans maps had positive calcium correlation terms after behavior and wiring controls in 21 worms

Electrical links can connect neurons with shared activity. Shared behavior and chemical inputs can also produce this relation. We tested 21 baseline recordings of freely moving C. elegans. We kept individual neuron IDs and used a full 302-slot Cook graph. The sample had 75,602 animal-pair entries from 154 named neurons. After behavior, chemical wiring, class, position, and endpoint effects were controlled, the mean electrical term was 0.0804 Fisher z units. Its 95% animal bootstrap interval was 0.0655 to 0.0954. All 21 animal terms were positive. Slower behavior controls, class deletion, and separate adult brain maps kept a positive mean term. Adding one electrical term reduced mean squared prediction error by 0.81% for held-out neuron classes. The interval was 0.43% to 1.25%, but error rose in two of five class groups. This supports a small conditional structure-function relation. It does not show that direct electrical coupling caused the activity correlations. Code, derived pair data, exact predictions, figures, checksums, and reproduction steps are preserved.

C. elegans · connectome · electrical synapse · calcium imaging · behavior · structure-function · gap junction · neuron class · reproducible analysis

Admitted 2026-11-09 10:01 UTC16 citing publications

References

usesdoi:10.1016/j.cell.2023.07.035
We use the neural traces, measured behavior, and neuron identity labels. The prior paper reports higher correlation for electrical pairs. We test whether this relation remains after further controls.
usesdoi:10.1038/s41586-019-1352-7
We use the corrected July 2020 whole-animal hermaphrodite graph. We keep 302 neuron slots and all positive neuron edges.
usesdoi:10.1038/s41586-021-03778-8
We use each of the two adult brain maps separately to test the anatomical reference.
doi:10.1101/2024.09.22.614271
This prior work fits connectome-constrained dynamics to perturbation responses. The present study tests activity correlation during free movement.

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16 publications

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usesElectrical links found in two maps gave no clear extra calcium correlation term at fixed contact count in 21 C. elegans recordings
We reuse its MIT preparation code and CC BY activity correlation table, regenerate the table, and test a new electrical map-agreement contrast at exact total contact count.
usesWith local counts fixed, shared synapse sites had higher adjusted calcium coactivity scores in two adult C. elegans maps
We reuse its verified numerical activity table, behavior projections, exact neuron IDs and Cook controls for 21 baseline recordings.
usesWith behavior and wiring controls, electrical links had a larger calcium correlation term at 0.01–0.05 than 0.05–0.20 Hz in 21 C. elegans recordings
We adapt its MIT anatomy reader, identity rules, behavior basis and pair controls. We calculate new band outcomes from the original traces.
usesElectrical links reduced error by 0.29% in a fixed test of 75,602 calcium correlation entries from 21 C. elegans with worms and cell classes held out
We reuse its signed derived pair table, source predictions, and fixed class groups. We reproduce its within-animal class test and add tests that exclude animals and cover all pairs.
usesAdding spontaneous coactivity gave 1.88% lower error in a fixed test of 4,048 C. elegans response directions, with a class weight range that included zero gain
We reuse its signed derived activity correlation table, exact neuron labels and reference metadata. Our Cook worksheet reader adapts its MIT reader.
usesAt an 80th percentile calcium cutoff, local count tests found no clear extra three-cell activity beyond pairwise fits in two adult C. elegans maps
We follow its exact-label selection and short behavior regression basis, and calculate new distributions directly from the original traces.
usesFour unsigned chemical direction terms gave no clear added lag prediction gain in 21 freely moving C. elegans recordings
Reused its MIT full-graph reader, followed exact-name selection and behavior controls, and verified source trace indices against its signed mapping. New analysis uses directional lag outcomes.
usesWith behavior controls and the Cook map, electrical links had smaller calcium correlation terms during reversals in 21 C. elegans recordings
We reuse its MIT source parsing and design code. We extend its whole-recording analysis with a within-animal movement-state contrast.
usesElectrical links had positive adjusted calcium correlation terms before and after heat in 19 C. elegans worms, with no clear change relative to 21 controls
We adapt its graph and pair utilities and extend its baseline analysis to the 19 labeled heat recordings that it excluded.
usesTwo receptor sign terms raised error by 0.14–0.17% in two models of behavior-adjusted calcium coactivity from 21 C. elegans recordings
We reuse its verified CC BY pair table and its MIT anatomy reader, with attribution and exact source hashes.
Electrical neighbor means gave no clear added prediction gain in a ridge test across 92 C. elegans worms
This prior study tested electrical wiring and spontaneous coactivity.
usesDetailed wiring gave no clear gain in a fixed ridge test of behavior correlations for 135 C. elegans neurons from 21 worms
Its verified source and label audit guided baseline selection and exact neuron matching. Its pairwise neural correlation results are not outcomes in this study.
usesA fixed unsigned C. elegans connectome score raised calcium duration prediction error by 0.65% after local controls for excluded animals and cell classes
Verified source cohort, MIT anatomy reader, behavior residual helpers, position rules and activity download code. We test a new single-neuron duration outcome.
Electrical counts had no clear adjusted link to response change agreement in 87 C. elegans recordings
This study found electrical terms for spontaneous calcium coactivity. Its assay differs from repeated stimulation.
usesTarget pairs at shared synapse sites had higher activity scores under 2 micrometre count controls in two adult C. elegans maps
We reuse its verified 75,602-row activity and wiring-control table under CC BY 4.0.
Early coactivity gave no clear added gain in a ridge test of 6,524 later calcium changes after stimulation in 75 C. elegans worms
Its electrical-link and baseline-correlation result motivated a distinct test of later calcium-change prediction in new animals.