With local counts fixed, shared synapse sites had higher adjusted calcium coactivity scores in two adult C. elegans maps

We tested whether target groups at chemical synapse sites carry information about calcium coactivity beyond weighted connections. We used all retained neuronal sites in two adult C. elegans brain maps and a fixed activity table from 21 freely moving worms. The common panel had 54,396 animal-pair rows, 8,038 named pairs and 134 neurons. All 180 graph labels entered the structural calculations. Random groups kept every connection count, site size and target count within 2 micrometre cubes fixed. Recorded groups had higher adjusted coactivity scores in both maps. Upper reference tails from 2,000 draws were 0.0040 and 0.0010; Holm values were 0.0040 and 0.0020. Shifting the spatial grid and merging records at the same source node kept positive gaps. A reference without spatial constraints gave no clear excess. Class weight ranges included zero in both maps. For classes excluded from training, adding the site score reduced mean error by 0.477% and 0.389%, with 95% animal bootstrap ranges of 0.077% to 0.907% and -0.139% to 1.139%. Only 13 and 10 of 21 animals improved. Thus the site groups carry a small conditional association in this panel. The result does not establish shared release or a general class effect. Both maps use the same functional data. The package preserves code, source hashes, graph summaries, the joined panel, predictions, random scores, figures and repeat steps. An offline replay and a full source rebuild checked the results.

connectome · polyadic synapse · shared site · calcium activity · C. elegans · structure and function · conditional randomization · reproducible benchmark