With local counts fixed, shared sites had closer transformed calcium responses in one of two adult C. elegans maps
We tested whether targets at the same chemical synapse site have similar calcium responses to activation of their common source. We used both adult Witvliet C. elegans brain maps and one fixed WT functional atlas. Each full neuronal map had 180 labels. The response test used 779 and 936 within-site target pair occurrences from 71 sources per map, with at least three finite trials per source-to-target response. The score was the mean squared difference between signed asinh responses. Random site assignments kept all connection counts, each site target count, and target counts within source, site-size group, and 2 micrometre cube fixed. In map 8, the score was 2.06% below the random mean. The lower-tail Monte Carlo value was 0.0030, or 0.0060 after Holm correction across the two primary tests. Map 7 gave no decrease. Merging records at the same source node reduced the map 8 decrease to 0.86%, with a lower-tail value of 0.0512. A five-trial cutoff gave 0.31% and 0.0544. Only 18 and 17 source scores varied under the local controls. These fixed-atlas results do not establish shared release or a general functional rule. The package preserves a plan, derived target-pair scores, all scalar random draws, code, figures, source hashes, and repeat steps. Six fresh downloads reproduced 49 numerical files. An exact 90-state example checked the sampler.
connectome · Caenorhabditis elegans · structure and function · polyadic synapse · calcium response · fixed margins · Curveball · synapse site
References
10- usesdoi:10.1038/s41586-021-03778-8
The native adult chemical synapse records, skeleton coordinates, and neuron definitions supply the anatomical input.
- usesdoi:10.1038/s41586-023-06683-4
The WT signal propagation atlas supplies signed calcium responses after activation of individual source neurons.
- usesdoi:10.17605/OSF.IO/E2SYT
Original functional data repository. The exact WormNeuroAtlas export and hash are recorded in the package.
- doi:10.1002/dneu.20307
Earlier neuromuscular experiments motivate the shared-site question. They do not establish the same mechanism for the brain test.
- usesTarget pair repeat counts exceeded random means with fixed edge and site counts in eight C. elegans brain maps sci:sha256:fd9de6f2f733031e639058d54b8184abce55a9ab7e3d549d16c86e8a5eb7b254
The earlier within-map site-group study supplies the fixed-count method and the original sampler code notice.
- usesTarget pair counts at birth had excess agreement with two adult C. elegans brain maps under local count controls sci:sha256:bd2ac676c8f1f8fb897c1c36ef64f853b3a8d668bcfd7de2f0b40fbd5c890cc8
We reuse its MIT C++ sampler without changes and use its site-count conventions. Our added test joins site groups to measured responses.
- usesAnatomy reduced ridge test error by 4.35% in wild type and increased it by 0.98% in unc-31 for 1,342 matched C. elegans atlas pairs sci:sha256:94b719902ca9f7ce3566f097497f5496034370c4e3341b81a5ffb5a82bbb6396
Its input audit guided finite-trial counts, exact name matching, and the response export provenance. No analysis code was copied.
- usesdoi:10.1038/ncomms5114
Curveball trades sample binary site memberships with fixed row and column counts.
- usesdoi:10.1103/PhysRevE.91.042812
The stationary law supports the fixed-count random reference distribution.
- usesdoi:10.1103/PhysRevE.94.039902
Correction to the Curveball stationary-law reference.
Cited by
3 publicationsPublic catalog entries on Sciscape.
- With local counts fixed, shared synapse sites had higher adjusted calcium coactivity scores in two adult C. elegans maps
This prior study tested shared sites against responses to source activation. Our study tests spontaneous target coactivity with a different functional dataset.
- usesIn a test between two adult C. elegans maps, shared sites gave no clear extra target connection score after contact and neuron controls
Its equal-site-size constraint informed an explicitly marked later sensitivity check. It tested calcium responses rather than the target connection outcome used here.
- usesTarget pairs at shared synapse sites had higher activity scores under 2 micrometre count controls in two adult C. elegans maps
We reuse its sampler and fixed local count design. Our outcome is spontaneous activity, not source-evoked response similarity.