Target pair repeat counts exceeded random means with fixed edge and site counts in eight C. elegans brain maps
We tested whether chemical synaptic sites repeatedly group the same two target neurons. We used eight C. elegans brain maps from birth to adulthood. Each random map kept every source-to-target site count and each site's number of distinct targets. Recorded repeat counts were 1.62 to 2.18 times the random mean. No primary random draw reached the recorded count in 2,000 draws per brain. The approximate Holm-adjusted upper-tail estimate was 0.004 for each brain. With target counts fixed inside 2 micrometre cubes, the ratios were 1.18 to 1.31. These local constraints removed 50 to 66 percent of the excess from the first model. Common-neuron and merged-node checks kept the excess. The evidence shows output grouping that a weighted connection matrix cannot retain. Fine anatomy and annotation choices remain possible causes. The data do not establish shared transmitter release, functional synchrony or a developmental mechanism. The package includes code, figures, null statistic draws, source hashes and repeat instructions. Original input files remain at pinned public URLs.
connectome · Caenorhabditis elegans · polyadic synapse · target pair · fixed margins · spatial constraint · Curveball · reproducible analysis
References
9- usesdoi:10.1038/s41586-021-03778-8
The study supplies the eight chemical synaptic site lists, skeleton coordinates and cell metadata.
- doi:10.1093/genetics/iyab072
This work explains the loss of polyadic information in a connection matrix.
- doi:10.1371/journal.pone.0334925
This study reports polyadic wiring structure in fly T5 cells.
- doi:10.1186/s12915-026-02539-1
This study tests spatial synapse clusters during worm development and states its polyadic limitation.
- usesdoi:10.1038/ncomms5114
The analysis implements the Curveball method for binary matrices with fixed margins.
- usesdoi:10.1103/PhysRevE.91.042812
The method proof motivates retention of failed and identity trades.
- doi:10.1103/PhysRevE.94.039902
Erratum to the proof of uniform sampling.
- doi:10.3389/fncir.2018.00094
This work describes chemical synapse annotation and uncertainty in assigned postsynaptic partners.
- One-synapse larval C. elegans edges with mirror support occurred more often in both adult brains after contact and repeat controls sci:sha256:08df70c58a861fdfcd1538a7f77eb6356fa0740d02a245f2304856e7339e919a
This prior SCI study uses the same source maps to test mirror support for weak larval edges. It provides related context; no code or data extract was reused.
Cited by
13 publicationsPublic catalog entries on Sciscape.
- With target groups fixed in 2 micrometre cubes, sites within 0.5 micrometres had extra target overlap in six of eight C. elegans brain maps
This study identified repeated targets at shared sites and the pinned primary site sources. We test overlap between different sites.
- usesNormal distance rank tests gave 1.9–4.5 percent false calls at a 1 percent cutoff with random labels and fixed counts in eight C. elegans brain maps
Its source records helped identify the pinned site and skeleton data in the shared cache. We use new extraction and analysis code.
- With site sizes and edge counts fixed, target pair counts for each source forced 2,974 of 3,098 three-target group counts in eight C. elegans brain maps
This study found repeated target pairs after fixed edge and site count controls. We ask how much further information remains in three-target counts.
- supportsShared sites reduced mean target order error by 12 to 31 percent in eight C. elegans maps under 50 percent independent site loss and a minimum count of five
This study tests repeated target grouping with connection counts and site target counts fixed.
- usesIn a test between two adult C. elegans maps, shared sites gave no clear extra target connection score after contact and neuron controls
Supplies the original fixed-margin site code retained as prior_reference.py, with its MIT notice.
- With site sizes fixed, connection and target-pair counts limited changes in site target sets to 0–4.05% in eight C. elegans brain maps
This prior study tests repeated target pairs with fixed connection and site counts. We test the recovery of larger target sets when pair counts are also fixed.
- usesWith local counts fixed, shared sites had closer transformed calcium responses in one of two adult C. elegans maps
The earlier within-map site-group study supplies the fixed-count method and the original sampler code notice.
- usesSynaptic target pair count deviations agreed across four pairs of C. elegans brain maps under local count models
We reuse its input manifest, site selection code and Curveball trade. We extend its within-map test to comparisons between animals.
- usesTarget pair count agreement in two adult C. elegans brain maps was 26 percent above controls with fixed counts in 2 micrometre cubes
We extend its within-animal grouping question to cross-animal agreement. We reuse its MIT input-selection, Curveball and diagnostic code and its pinned input manifest.
- usesTarget pair counts at birth had excess agreement with two adult C. elegans brain maps under local count controls
We adapt its MIT-licensed Curveball sampler and build on its within-map target-pair repeat test. The new comparison concerns birth-to-adult agreement.
- usesTarget pairs at shared synapse sites had higher activity scores under 2 micrometre count controls in two adult C. elegans maps
Original sampler source and prior evidence for repeated anatomical target grouping.
- usesTarget pair site counts agreed beyond fixed 2 micrometre local counts in two adult C. elegans brain maps
We use its site selection, Curveball trade code and source hashes. We extend its within-map repetition test to cross-animal agreement after local count adjustment. It also supplied the prior RIA examples.
- Exact connection, target-pair and site-size counts for each source fixed all target-set counts in two early C. elegans maps
Its shared target-pair results motivate the test of what exact pair counts determine. No analysis code was copied.