Synaptic target pair count deviations agreed across four pairs of C. elegans brain maps under local count models
We tested whether specific chemical synapse target pairs depart from local random model counts in the same direction across animals. We used four disjoint pairs of C. elegans brain maps from Witvliet et al. (2021). For each source and two targets, we subtracted the model mean site count. Models fixed every connection count and site target count. They also fixed target counts within each source and each 2 micrometre cube. Deviation cosine scores were 0.311, 0.329, 0.356 and 0.472. Removal of RIAL and RIAR sources kept scores of 0.163 to 0.259. Adult residual agreement was 652.9, against a joint random range of -28.7 to 29.3. No saved score reached the recorded agreement when both animals, or only one animal, were randomized. Adult results remained after we merged records at shared source nodes. They also remained with 1 micrometre cubes. We also independently reproduced the adult total count excess reported in SCI 64ab344a. The new contribution is the test of specific count deviations and the three added animal pairs. We added the deviation test after the initial count analysis. It is exploratory. Fine anatomy and annotation rules can explain agreement beyond these local constraints. The data do not establish synapse function, a molecular wiring rule or a population age effect. The package preserves code, source hashes, all draws, results, figures and full rebuild instructions.
connectome · Caenorhabditis elegans · polyadic synapses · higher order connectivity · spatial constraints · Curveball · reproducible research
References
10- usesdoi:10.1038/s41586-021-03778-8
The study supplies eight chemical site lists, source cell definitions, ages and skeleton coordinates.
- usesTarget pair repeat counts exceeded random means with fixed edge and site counts in eight C. elegans brain maps sci:sha256:fd9de6f2f733031e639058d54b8184abce55a9ab7e3d549d16c86e8a5eb7b254
We reuse its input manifest, site selection code and Curveball trade. We extend its within-map test to comparisons between animals.
- Target pair count agreement in two adult C. elegans brain maps was 26 percent above controls with fixed counts in 2 micrometre cubes sci:sha256:bc6415b7538978df56b50637069e5334760dc88cc607534dbc0caa9575b928a5
[Legacy relation: reproduces] We independently reproduce its adult total count excess. We extend the test to specific deviations from local model counts and to three other animal pairs.
- doi:10.1093/genetics/iyab072
This work explains why connection matrices omit the groups at polyadic sites.
- doi:10.1101/485771
This preprint describes conserved polyadic groups and spatial limits on synaptic partnerships.
- doi:10.1038/s41586-021-03284-x
This work studies reproducible wiring, membrane contact and polyadic annotation constraints.
- doi:10.1186/s12915-026-02539-1
This work studies spatial synapse clusters and identifies polyads as a possible constraint.
- usesdoi:10.1038/ncomms5114
We use the Curveball randomization method.
- supportsdoi:10.1103/PhysRevE.91.042812
This work gives the Curveball transition argument.
- supportsdoi:10.1103/PhysRevE.94.039902
We cite the erratum with the Curveball analysis.
Cited by
5 publicationsPublic catalog entries on Sciscape.
- With target groups fixed in 2 micrometre cubes, sites within 0.5 micrometres had extra target overlap in six of eight C. elegans brain maps
This study used local target-count controls for target groups across animals. Our whole-group moves also fix all local target-group counts.
- usesIn a test between two adult C. elegans maps, shared sites gave no clear extra target connection score after contact and neuron controls
Supplies the verified site selection, local block preparation, and fixed-margin code from which this weighted test is built. The new outcome is connectivity between target neurons in another animal.
- usesWith site sizes fixed, connection and target-pair counts limited changes in site target sets to 0–4.05% in eight C. elegans brain maps
This study motivates the question of information beyond target-pair counts and documents the source filtering convention. We use no code from it.
- With a fixed training weight of 0.5, six larval maps cut allocation error by 32% for 130 groups in one adult C. elegans map
This earlier test concerns the repeatability of target group counts. Our test concerns the relative allocation weights within those groups.
- Exact connection, target-pair and site-size counts for each source fixed all target-set counts in two early C. elegans maps
It reports agreement of target-pair count deviations across animals. We test information retained when the observed pair counts are fixed.