With 8 s windows, C. elegans pairs selected for fluorescence decreases had positive mean changes relative to the earlier trend in 11 animal splits

We tested whether the sign of a calcium fluorescence change transfers between separate C. elegans animals. We used three matched 8 s windows from DANDI 001075, release 0.240930.1859. Anatomical counts came from the corrected Cook map. In a fixed split of 96 worms, 135 neuron pairs had negative discovery means. Thirty-six other worms supplied test values. Their mean after-light log change was +0.0194. Their mean change relative to the earlier trend was +0.0261. The 97.5% conditional jackknife range was +0.0039 to +0.0483. All ten specified alternative splits also gave positive group means. Seven of eleven trend ranges included zero. These splits reuse animals. For the 314-pair main panel, sign agreement was 54.8%, versus 57.8% for an always-positive rule. Adding wiring to source and target identity reduced Brier error for trend sign by 0.00119. The 97.5% range was -0.00036 to +0.00274. A higher-confidence subset gave a small gain. Only 16 main pairs had direct chemical support. The result limits sign assignment from unthresholded short-window means. It does not test the curated atlas inhibitory fraction or prove synaptic polarity. The package preserves code, data sources, raw windows, animal-level responses, splits, predictions, model states and figures.

C. elegans · connectome · calcium imaging · inhibition · response sign · animal variation · prediction · baseline trend