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q-bio.NC · Neurons and Cognitionq-bio.QM · Quantitative Methods

Centered source contrasts agreed across repeats in a fixed calcium test of 45 C. elegans worms

We tested whether target response patterns distinguish two stimulated sources on separate repeats within one C. elegans worm. The fixed panel has 362 four-event blocks, 368 distinct stimuli and 12,906 distinct target measurements in 45 worms. Source differences and target centering cancel additive target and event terms in a transformed green-minus-red calcium measure. The mean repeat cross product was 0.1826, with a central 95% complete-worm resampling range of 0.1129 to 0.2708. Normalized agreement was 0.197. All planned post-response selection checks kept positive agreement. A later matched pre-stimulus control gave a post-minus-before score of 0.1677, with a range of 0.0947 to 0.2488. Direct chemical and electrical count profiles had positive alignment after cubic soma distance control. Its excess above a later geometric reference of equal rank was 0.0050, with a range of -0.0028 to 0.0135. Its post-minus-before range also crossed zero. The test thus found repeat agreement without clear added direct-count specificity under these controls. Reading the cross product as stable signal energy requires stable means and zero cross-event error covariance. State, history, gains and optical effects can violate these assumptions. The package has source hashes, exact input facts, new pre-channel facts, all scores, code, figures and repeat steps.

C. elegans · connectome · structure and function · calcium imaging · repeat agreement · source contrast · optogenetics · noise correction · soma distance · open data

Admitted 2 citing publications

References

8
  1. usesAnatomical paths reduced mean calcium prediction error in 103 C. elegans animals excluded from training sci:sha256:a10115ec5ae0e7b414c52276d1b6d39d2529e9168b00f3fed9ac6719192f7206

    We reuse its verified event and subject facts, complete anatomical arrays, and MIT XML anatomy checker.

  2. Count differences between two adult maps gave no clear gain in a ridge test of response spread across worms for 370 C. elegans neuron pairs sci:sha256:86ab890b18acc5cfc2be4513f36320cb0e6ac5a0db4e65bea0889d93abea7fbc

    This earlier study includes repeated-response variance components. We test centered source contrasts across a common target panel.

  3. Assuming independent animals and stable means, a cubic soma distance control reduced the estimated wiring overlap by 83% in 99 C. elegans worms sci:sha256:b79bb1d12567da2a4517745418d690f33a702bab98c5bf2adf177415a5f002a6

    This earlier study motivates soma distance controls and stable response products. Its cross-animal task differs from this test.

  4. usesdoi:10.1038/s41586-023-06683-4

    Original single-neuron stimulation and calcium measurements. The original study already reports variability and kernel similarity.

  5. usesdoi:10.48324/dandi.001075/0.240930.1859

    Fixed CC BY 4.0 release of all 110 processed NWB records. Every raw file hash was checked.

  6. usesdoi:10.1038/s41586-019-1352-7

    Corrected July 2020 Cook hermaphrodite chemical and electrical section-count maps.

  7. doi:10.1016/j.neuroimage.2015.12.012

    Established independent-partition distance principle. The crossed-product method is not claimed as a new general estimator.

  8. doi:10.1371/journal.pcbi.1009212

    Established treatment of noise bias and limits in neural model comparisons.

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