Assuming independent animals and stable means, a cubic soma distance control reduced the estimated wiring overlap by 83% in 99 C. elegans worms
We tested how anatomical wiring aligns with calcium response means after neuron and soma distance terms. We used 1,967 directed neuron pairs from 99 wild-type C. elegans worms. Products from different animals estimate squared mean components if animals are independent and direction means are stable. After source and target terms, nine fixed wiring features gave a component of 0.007510, or 24.6% of the estimated stable pair residual. A later cubic soma distance control reduced this component to 0.001295. The decrease was 82.8%, with an approximate 95% animal jackknife range of 71.5 to 94.0%. This distance analysis was exploratory. After distance control, the post-minus-before wiring component was 0.001159, with a range of -0.000104 to 0.002422. Excluding products from the same recording day left the component before distance control nearly unchanged. Linear and spline distance checks also reduced it. These are fixed subspace moments, not held-out prediction scores or causal synaptic effects. The package preserves code, event facts, graph inputs, source versions and hashes, all estimates and deletions, simulations, figures and repeat instructions.
connectome · C. elegans · calcium imaging · soma distance · sampling variation · response means · structure and function
References
9- usesAnatomical paths reduced mean calcium prediction error in 103 C. elegans animals excluded from training sci:sha256:a10115ec5ae0e7b414c52276d1b6d39d2529e9168b00f3fed9ac6719192f7206
We reuse and verify the animal-labelled response facts, subject metadata and complete neuronal graph.
- usesProducts from different animals cut the mean squared direction difference estimate by 94% in 1,759 C. elegans pairs sci:sha256:abd3696cc86b171e261361896a398eb58ef643707dc15553d9666e8052effa26
We use its established formula for response products from different animal identities. This study tests the full directed panel and distance controls.
- usesNeuron terms left 86% of the expected cross product between random halves of 6,270 C. elegans response lists after an asinh transform sci:sha256:e2fcb76e66bbd3b6fc0a95dde6d955b0b33aaac2fec9f7b4953d9e5846c5dbf1
Its fixed-list subspace analysis motivates the distinct-animal analysis. We do not infer animal stability from random trial halves.
- usesNine wiring terms gave no clear gain in a fixed prediction test of same-side response contrasts in 1,381 C. elegans class pairs sci:sha256:41b15854cd8523c5ffd0b63681f8b54798c97d57f265ff77870b2e99ead7941f
The verified input study credits this work for graph facts and the nine structural path definitions.
- usesdoi:10.1038/s41586-023-06683-4
Original single-neuron stimulation and calcium recordings.
- usesdoi:10.48324/dandi.001075/0.240930.1859
Fixed source release for the preserved derived response facts.
- usesdoi:10.1038/s41586-019-1352-7
Cook whole-animal neuronal anatomy, corrected July 2020 workbook.
- supportsdoi:10.1371/journal.pcbi.1009212
Established noise-corrected model comparison and limits of low-signal ratios.
- supportsdoi:10.1016/j.neuroimage.2015.12.012
Established independent-partition quadratic distance principle.
Cited by
3 publicationsPublic catalog entries on Sciscape.
- usesGreen and green/red rules gave opposite response signs in 18.2% of 48,300 measurements from 103 C. elegans recordings
We build on its use of different-animal response products and fixed neuron and distance subspaces. Our new test compares paired reference-channel rules.
- Centered source contrasts agreed across repeats in a fixed calcium test of 45 C. elegans worms
This earlier study motivates soma distance controls and stable response products. Its cross-animal task differs from this test.
- supportsSoma distance predicted detected connections in the C. elegans signal propagation atlas more than EM wiring, and near responses followed in-plane image distance within neuron pairs
That study found that a soma distance control removes most of a wiring component; we show that soma distance predicts detection more than wiring does.