Green and green/red rules gave opposite response signs in 18.2% of 48,300 measurements from 103 C. elegans recordings
We tested how a red reference changes a calcium response measure used in connectome studies. We compared green-only and framewise green/red response rules on the same 48,300 selected event-target measurements from 103 C. elegans recordings. The rules gave opposite observed signs in 8,801 measurements (18.22%). When both changes were at least 5% in magnitude, 585 of 17,976 measurements had opposite signs (3.25%). We then used products from different animals for 5,320 directed pairs with at least three animals each. Under independent animals and stable selected-pair means, these products estimate squared response-mean components without same-animal products. After neuron and cubic soma-distance controls, the estimated overlap with 11 unsigned wiring features was 0.002406 for green only and 0.002212 for green/red. The paired change was -0.000194, with a normal animal-jackknife range from -0.000905 to 0.000517. This range does not establish equivalence. An added audit on the same valid frames retained 8,800 sign disagreements. The study shows sensitivity of observed window signs to the response rule. It does not identify which rule better measures neural activity. The package preserves channel summaries, code, exact source records, all specified checks, figures and repeat instructions.
C. elegans · connectome · calcium imaging · reference channel · response sign · animal replication
References
9- usesWiring features reduced calcium response error by 1.17% beyond cell identity in a fixed test across 103 C. elegans worms sci:sha256:0e5e26303629eecdd516b6e9419e39fd70425547e36bb717a69612e587e68861
We reuse its exact event table, Cook graph, 11 wiring features and MIT extraction code, and rebuild them from source.
- usesAfter identity, pre-stimulus signal and distance controls, nine unsigned wiring terms gave no clear target selection gain in a ridge test of 1,225 stimuli from 103 C. elegans worms sci:sha256:286b9f54ffb3ca53ccb485591b6f0b43101e0711383e16e8a2cf66854fdd816b
We reuse its 302-row template coordinate arrangement and source provenance.
- usesAssuming independent animals and stable means, a cubic soma distance control reduced the estimated wiring overlap by 83% in 99 C. elegans worms sci:sha256:b79bb1d12567da2a4517745418d690f33a702bab98c5bf2adf177415a5f002a6
We build on its use of different-animal response products and fixed neuron and distance subspaces. Our new test compares paired reference-channel rules.
- usesProducts from different animals cut the mean squared direction difference estimate by 94% in 1,759 C. elegans pairs sci:sha256:abd3696cc86b171e261361896a398eb58ef643707dc15553d9666e8052effa26
This prior study motivates the removal of same-animal products when estimating repeated response components.
- Five image position terms removed a 0.37% wiring gain in a fixed test of 1,225 C. elegans stimuli sci:sha256:696624f718fdadc24ed3ca7236e3cda00b94be6a43a6845907ed614523df82d2
This prior prediction study already found a role for image position controls. We do not claim that result as new.
- Soma distance predicted detected connections in the C. elegans signal propagation atlas more than EM wiring, and near responses followed in-plane image distance within neuron pairs sci:sha256:30698592c6fd42752f488ca2d57b112feb1cdb3fc7b5a7501d42f83a9a48da7a
This prior study measured distance and optical-axis effects in the assay. The present readout comparison does not identify an optical cause.
- usesdoi:10.1038/s41586-023-06683-4
Original calcium and stimulation measurements by Randi, Sharma, Dvali and Leifer.
- usesdoi:10.48324/dandi.001075/0.240930.1859
Pinned CC BY 4.0 NWB data release, with conversion by CatalystNeuro.
- usesdoi:10.1038/s41586-019-1352-7
Original chemical and electrical anatomy from Cook, Jarrell and colleagues; corrected July 2020 workbook.
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