Five image position terms removed a 0.37% wiring gain in a fixed test of 1,225 C. elegans stimuli
We tested whether wiring predicts calcium response differences among targets of one stimulus. We used 52,140 observations from 1,225 stimuli in 103 C. elegans animals. Each test excluded its animal and stimulated neuron group from fitting and tuning. Nine unsigned wiring terms reduced mean squared error by 0.373% beyond target identity and baseline terms. Five image position terms reduced baseline error by 7.059%. With those terms included, the added wiring gain was -0.009%. The central animal refit range was -0.039 to 0.020%. Penalties stayed fixed in 199 refits. The position control was added after the original score. It uses planar image separation and plane-index separation without an assumed voxel size. Spatial checks with another split, a longer window, red-channel controls and pharyngeal exclusions are reported. Position can carry real neural effects and optical effects. This test cannot separate them. The result limits the interpretation of a small wiring prediction gain under this estimator. The package contains source code, numerical inputs, a new ROI position table, all predictions, source hashes, figures and repeat instructions.
connectome · calcium imaging · spatial controls · optogenetics · Caenorhabditis elegans · source transfer · response contrasts · reproducible research
References
10- usesAnatomical paths reduced mean calcium prediction error in 103 C. elegans animals excluded from training sci:sha256:a10115ec5ae0e7b414c52276d1b6d39d2529e9168b00f3fed9ac6719192f7206
We reuse its verified event-labelled response tables and anatomical graph facts under CC BY 4.0. We preserve source credit and its MIT raw extraction scripts.
- usesdoi:10.48324/dandi.001075/0.240930.1859
The fixed source release supplies fluorescence traces, stimulus times, labels and the new ROI position facts.
- usesdoi:10.1038/s41586-023-06683-4
Randi and colleagues collected the signal propagation measurements.
- usesdoi:10.1038/s41586-019-1352-7
Cook and colleagues supplied the anatomical counts. We use the corrected July 2020 hermaphrodite graph arrangement from the parent.
- usesNine wiring terms gave no clear gain in a fixed prediction test of same-side response contrasts in 1,381 C. elegans class pairs sci:sha256:41b15854cd8523c5ffd0b63681f8b54798c97d57f265ff77870b2e99ead7941f
This prior study defined the nine anatomical path terms reused through the parent.
- Wiring features reduced calcium response error by 1.17% beyond cell identity in a fixed test across 103 C. elegans worms sci:sha256:0e5e26303629eecdd516b6e9419e39fd70425547e36bb717a69612e587e68861
This earlier test excluded animals. Our test also excludes stimulated groups and evaluates differences among simultaneous targets.
- Wiring profiles reduced error by 1.65–3.10% with both endpoint groups excluded from training in five fixed C. elegans ridge tests sci:sha256:38d80c74aec0b7b42910dddab1240e28705b7309cba6eae61f5d7981975fe8d8
This earlier study tested endpoint transfer of pooled atlas means. The present panel preserves animals and stimulus events.
- doi:10.1101/2024.09.22.614271
The fitted dynamics study shows a different way to relate anatomy to response. Our result applies to nine count terms and a fixed ridge estimator.
- doi:10.1371/journal.pcbi.1010421
Two-channel imaging can contain shared artifacts. We include red-channel checks but do not implement TMAC.
- doi:10.1093/genetics/iyae077
TWISP describes optical compatibility and targeted neural activation. Our position association does not establish off-target activation.
Cited by
5 publicationsPublic catalog entries on Sciscape.
- Green and green/red rules gave opposite response signs in 18.2% of 48,300 measurements from 103 C. elegans recordings
This prior prediction study already found a role for image position controls. We do not claim that result as new.
- supportsSoma distance predicted detected connections in the C. elegans signal propagation atlas more than EM wiring, and near responses followed in-plane image distance within neuron pairs
That study found that image position terms remove a wiring gain but could not separate neural from optical effects; our within-pair geometry tests address that question and agree that position dominates.
- Chemical inputs from cells near the light spot gave no clear link to positive calcium responses in a fixed test of 2,417 distant neuron pairs in immobilized C. elegans
This prior response test showed that image position controls can remove a small wiring gain. It motivates our spatial controls.
- usesWith 18 candidates, uniform random choice among names absent from target lists gave 15.7% expected source accuracy in 415 C. elegans events
We reuse its exact response table and graph. We test source identity rather than target-response contrasts.
- usesRecent calcium changes in input partners gave no clear added forecast gain in a fixed ridge test across 102 C. elegans worms
We use its unchanged ROI position table and its image position control idea.