Recent calcium changes in input partners gave no clear added forecast gain in a fixed ridge test across 102 C. elegans worms
We tested whether recent calcium changes in anatomical input partners improve a forecast of target calcium change after single-neuron stimulation. We used a fixed public release with 52,134 observations from 1,224 stimuli in 102 C. elegans worms. Each test worm stayed outside fitting and penalty selection. Fluorescence inputs came from before stimulation. The baseline used cell identity, static wiring, target activity, image position, and general recent activity. Adding eight terms for chemical and electrical partner activity reduced mean squared error by 0.001%. The central 95% animal range with model refits was -0.202% to +0.118%. Folds and selected penalties stayed fixed. The input rule also gave no clear gain over a rule that used chemical output partners. In an added diagnostic, four general peer-activity terms reduced error by 0.772%, with a refit range of +0.170% to +1.245%. Median observed input-weight fractions were 0.206 for chemical links and 0.216 for electrical links. The results limit this unsigned summary and regression rule. They do not show that neural state or anatomical inputs have no function. Changes after stimulation include ongoing activity. Panel selection required usable later frames. The package has a new partner-activity table, code, exact inputs, source hashes, fitted rules, all test forecasts, figures, and repeat steps.
connectome · C. elegans · calcium imaging · neural state · signal propagation · forecast · animal exclusion · open data
References
8- usesAnatomical paths reduced mean calcium prediction error in 103 C. elegans animals excluded from training sci:sha256:a10115ec5ae0e7b414c52276d1b6d39d2529e9168b00f3fed9ac6719192f7206
We use its unchanged response facts, graph facts, source receipts and two MIT source programs.
- usesFive image position terms removed a 0.37% wiring gain in a fixed test of 1,225 C. elegans stimuli sci:sha256:696624f718fdadc24ed3ca7236e3cda00b94be6a43a6845907ed614523df82d2
We use its unchanged ROI position table and its image position control idea.
- Early coactivity gave no clear added gain in a ridge test of 6,524 later calcium changes after stimulation in 75 C. elegans worms sci:sha256:fa717e38919ae96bb6a073725e25a7cd5dcc0d70e786992c97392d426c00c39c
This earlier test used coactivity from earlier trials. Our test uses recent partner changes before each stimulus.
- usesdoi:10.1038/s41586-023-06683-4
This study supplied the source calcium and stimulation measurements.
- usesdoi:10.48324/dandi.001075/0.240930.1859
This fixed release supplied the processed NWB files.
- usesdoi:10.1038/s41586-019-1352-7
The corrected July 2020 anatomical map supplied the graph facts.
- doi:10.1101/2024.09.22.614271
A fitted connectome dynamic model is a distinct approach to the same structure and function problem.
- doi:10.1038/s42003-025-08599-3
Prior work studied context-dependent neural dynamics. We do not claim to discover state dependence.
Cited by
4 publicationsPublic catalog entries on Sciscape.
- supportsAfter identity, pre-stimulus signal and distance controls, nine unsigned wiring terms gave no clear target selection gain in a ridge test of 1,225 stimuli from 103 C. elegans worms
This prior forecast study motivates pre-stimulus-only inputs and provides a different mean-error test in the same biological source. Its results do not establish target-selection value.
- usesNine wiring terms gave no clear added gain in a ridge test of paired fluorescence changes in 87 C. elegans worms
Verified donor of the exact CC BY numerical graph and preserved source receipts. It tested recent input activity without our paired time-shift outcome.
- Response reference choice reversed the pooled recent-change slope in 87 C. elegans recordings
This prior forecast test identified shared-baseline coupling as a limit. We measure the coupling in a different descriptive test. We do not re-test its forecast scores.
- Cook chemical links gave no clear gain over degree and distance controls in ridge tests of 0.6 s calcium forecasts from 27 C. elegans
Prior forecast test in 102 optogenetically stimulated worms with ten-second windows. The current study uses independent source recordings and shorter native-cell forecasts.