Products from different animals cut the mean squared direction difference estimate by 94% in 1,759 C. elegans pairs
We tested whether calcium response differences between opposite stimulation directions repeat across animals. We used fixed event summaries from DANDI 001075 version 0.240930.1859. The panel had 1,759 neuron pairs and 101 WT worms, with at least three animals per direction. We formed response products only between different animal identities. Under independent animals and stable direction means, these products estimate squared population means without the usual sampling term. The mean squared half difference fell from 0.054330 for squared sample means to 0.003365 for products from different animals, a 93.8% decrease. Its approximate 95% animal jackknife range was -0.005679 to 0.012409. The common response energy was 0.043218, with a range of 0.026020 to 0.060416. All five specified checks gave directional ranges that included zero. A fixed graph projection left uncertain endpoint and cycle components. A fixed direct chemical count contrast also had an alignment range that included zero. These results limit tests that use directional response summaries as prediction targets. They do not show biological symmetry or define a neural noise ceiling. The stable mean and animal sampling assumptions can fail. Before-pulse changes also had common energy, which limits a causal reading. The package includes code, derived data, exact source records, figures, all animal deletions, and repeat steps. A full raw-data rebuild matched the event and subject tables byte for byte.
connectome · Caenorhabditis elegans · signal propagation · directional response · animal sampling · second moments · Hodge decomposition
References
9- usesAnatomical paths reduced mean calcium prediction error in 103 C. elegans animals excluded from training sci:sha256:a10115ec5ae0e7b414c52276d1b6d39d2529e9168b00f3fed9ac6719192f7206
We reuse and verify the event and subject tables, anatomical facts, MIT extraction and source-check code, and source records.
- usesdoi:10.48324/dandi.001075/0.240930.1859
Fixed source release for all raw fluorescence traces, neuron labels and stimulation records.
- usesdoi:10.1038/s41586-023-06683-4
Original single-neuron stimulation and whole-brain calcium measurements.
- usesdoi:10.1038/s41586-019-1352-7
Original anatomical source; the chemical matrix is from the workbook corrected July 2020.
- Neuron terms left 86% of the expected cross product between random halves of 6,270 C. elegans response lists after an asinh transform sci:sha256:e2fcb76e66bbd3b6fc0a95dde6d955b0b33aaac2fec9f7b4953d9e5846c5dbf1
Related fixed-list cross-product analysis lacked animal labels. This study uses distinct animal identities for all second moments.
- One stored trial value could reverse the larger-response direction in 147 of 310 C. elegans pairs with unequal chemical counts sci:sha256:cac8bdd8bfe7569f321bd938577f8d12c6cea90cdc4df98c2f37b410ba6728f6
Prior direction-order sensitivity analysis states the need for independent biological samples.
- Wiring profile gains changed sign across five ridge splits of two-way response differences in 1,855 C. elegans atlas pairs sci:sha256:a144ac954d855cb9241b2cb125b4e37046526ac51aeaceff51be0aeb124eddb4
Prior wiring-profile tests gave unstable gains for opposite-direction response differences.
- doi:10.1016/j.neuroimage.2015.12.012
Established principle of using independent response partitions to remove sampling bias from squared differences.
- doi:10.1007/s10107-010-0419-x
Established graph Hodge projection into endpoint gradients and cycle components.
Cited by
4 publicationsPublic catalog entries on Sciscape.
- usesGreen and green/red rules gave opposite response signs in 18.2% of 48,300 measurements from 103 C. elegans recordings
This prior study motivates the removal of same-animal products when estimating repeated response components.
- usesWith fixed means and independent errors of finite variance, repeat products give unbiased squared three-cell loop estimates
Its independent-product correction for direction differences motivates the loop correction; we do not reuse its outcomes as new results.
- Repeat correction cut mean squared contrast distance by 83% in 22 C. elegans worms with matched sources and targets
This prior study used products from different animals to estimate squared population differences between opposite stimulation directions. Our target is the difference between matched animal source contrasts.
- usesAssuming independent animals and stable means, a cubic soma distance control reduced the estimated wiring overlap by 83% in 99 C. elegans worms
We use its established formula for response products from different animal identities. This study tests the full directed panel and distance controls.