In one V1DD mouse, mean synapse size had no clear relation to movie response similarity in 1,441 connections after cell, count and geometry controls
We tested whether larger mean synapse size joins cells with more similar movie responses. We used 1,441 connected excitatory cell pairs from one V1 Deep Dive mouse. The model controlled source and target cell identity, synapse count, proximity and soma distance. The primary outcome used deconvolved calcium events from separate movie repeat groups. A doubling of mean size changed response correlation by 0.00021. The 95% dyadic interval was -0.00571 to 0.00613. All planned V1DD sensitivity intervals included zero. A neuron resample also spanned zero. A reference test used 2,840 measured V1-to-V1 connections from the MICrONS mouse. It reproduced a positive relation with geometry, count and source controls. Adding target controls kept a positive estimate but gave a wider interval that included zero. Many reference targets had only one connection in the selected table. The result concerns response similarity among selected connections. It does not measure synaptic efficacy or show no relation between structure and function. Two mice with different protocols cannot establish an animal-population difference. The package includes selected data, exact source versions, checksums, code, figures and repeat instructions.
connectome · structure and function · synapse size · V1 Deep Dive · MICrONS · visual cortex · calcium imaging · cell controls · dyadic data · reproducible analysis
References
4- usesIn the V1 Deep Dive mouse, connected excitatory neurons had only slightly more similar movie responses than nearby unconnected neurons; the same test gave a 2 to 7 times larger effect in MICrONS sci:sha256:dd15d6d8156d77ee6c2bf3f36180346ce5d8f7d0ceecd54d2e329703851a4469
We use its verified V1DD response and anatomy tables. We add a mean synapse size test with source and target cell controls.
- usesdoi:10.1038/s41586-025-08840-3
We use the release v1 node and edge tables for a measured MICrONS reference test.
- usesdoi:10.1038/s41586-025-08790-w
This study describes the source MICrONS functional connectome.
- usesdoi:10.1093/pan/mpv018
We use dyadic cluster variance to allow correlated errors among connections that share a cell.
Cited by
3 publicationsPublic catalog entries on Sciscape.
- Measured movie similarity gave no clear added wiring gain over model similarity with test neurons excluded in one MICrONS mouse
This prior test links measured response similarity to synapse size. It provides related source checks. Our test instead compares nested wiring classifiers with test neuron exclusion.
- Recorded synapses gave a 0.025% error decrease beyond a digital twin in a calibration test of 91,647 MICrONS V1 pairs
Prior SCI work tests mean synapse size with cell, count and geometry controls. It motivates a separate prediction test with model response similarity given.
- usesRemoving connections with no size slope information reduced an HC1 variance estimate by 53% and 9% in two mouse cortex models
We reuse its exact selected data and fixed model code. We quantify its global HC1 correction and test calibration on its two graph designs.