Mirror counts improved selection of half the chemical links among paired neurons in three adult C. elegans brains
We tested whether a mirror connection adds information about chemical connection presence in another adult brain. We used 166 neurons in 83 left and right pairs. Adult records 7 and 8 came from Witvliet et al. N2U supplied a third adult target. At one source synapse record, connections with a mirror occurred in the other adult in 194 of 313 cases. Connections without a mirror occurred in 120 of 431 cases. The reverse comparison gave 185 of 309 and 125 of 402. We ranked observed source links by their own count or by own count plus mirror count. Each method kept floor(N/2) links. Only source data set the ranks. Uniform cutoff ties had exact expected weights. Precision rose from 88.16% to 91.67% for 7 to 8, and from 87.01% to 90.92% in reverse. For N2U targets, it rose from 83.59% to 87.68% and from 84.63% to 87.29%. The half-size gain stayed positive under the stated counting, cell, and target-threshold checks. It also stayed positive after each neuron pair was removed in turn. A separate audit reproduced the main results with rational arithmetic. A fresh download reproduced all 17 numerical output files. The package preserves code, exact source versions, hashes, candidate tables, selection weights, figures, and reproduction commands. This exploratory benchmark has three animals and four comparisons that share animals. It does not estimate a population effect or separate biological variation from annotation variation. The pooled score can lose a small amount at some smaller selection sizes.
C. elegans · connectome · bilateral symmetry · chemical synapse · connection selection · reproducibility · synapse count · adult brain
References
6- usesdoi:10.1038/s41586-021-03778-8
We use adult synapse records 7 and 8, neuron labels, a contact matrix, and a legacy table from the pinned source repository.
- usesdoi:10.1038/s41586-019-1352-7
The secondary target uses the N2U chemical connection reannotation, as supplied by Witvliet et al.
- usesdoi:10.1098/rstb.1986.0056
N2U comes from this adult animal. Its reannotation is one target animal, rather than a new animal for each paper.
- doi:10.1038/s41586-021-03284-x
This prior study used bilateral and inter-animal recurrence to define a reference circuit. It provides the context for the present count-controlled selection benchmark.
- doi:10.1111/ejn.70629
This prior study tests shared network roles of bilateral neurons. The present benchmark tests connection occurrence in another animal at fixed source count and selection size.
- supersedesMirror counts improved selection of half the chemical links among paired neurons in three adult C. elegans brains sci:sha256:9416e2cdfe153e4bd911468f358783e23eb52a020e8f1163c24a385b82680b98
This record corrects one provenance date label. The inputs were retrieved on 28 October 2026 UTC, which was 27 October locally. Source hashes, numerical files, code, figures, and conclusions are unchanged.
Cited by
2 publicationsPublic catalog entries on Sciscape.
- Mirror synapse counts did not lower response detection error in 9,725 C. elegans atlas pairs under a fixed logistic model
This study found useful mirror counts for predicting repeated anatomy. We test a different outcome: detected signal propagation.
- usesSynapse volume raised adult match rates when count ties were broken to keep half the chemical edges in four early C. elegans maps
We use its sum of own and mirror counts as a secondary selection score. We test added volume tie breaks.