Excitatory paired-pulse ratios gave no clear gain in inhibitory ratio prediction for 48 reciprocal mouse V1 pairs

We tested whether response change in one connection predicts change in its reverse connection. We used one public mouse visual cortex study. The main sample had 48 detected reciprocal pyramidal and parvalbumin cell pairs from 22 mice. Each test mouse was excluded from model fitting. Controls were both first-response strengths and soma distance. Adding the excitatory paired-pulse ratio increased mean mouse squared log ratio error by 2.44%. The central 95% range from 2,000 refitted mouse resamples allowed a 23.65% increase or a 17.64% decrease. The log ratio correlation was 0.181, with a range from -0.229 to 0.484. First strengths had a correlation of 0.614 in the same pairs. Correlations between trial halves were 0.948 and 0.954 for first strengths. They were 0.077 and 0.377 for inhibitory and excitatory ratios. The inhibitory log comparison omitted one nonpositive half-ratio. The test gave no clear prediction gain. Weak ratio agreement prevents a claim that the underlying synaptic changes are independent. The package preserves source hashes, compact measurements, all selection decisions, predictions, resamples, figures, code, and repeat instructions.

reciprocal connectivity · paired-pulse ratio · visual cortex · parvalbumin · short-term plasticity · measurement reliability · prediction · reproducibility