The 12 source neuron traces do not give center error bounds for 79 segment and radius pairs.

We examined whether the Allen Cell Types Database records provide measured soma-center error bounds for 79 Sholl segment and radius pairs in 12 mouse neuron traces. The examined source comments, SWC columns, soma rows, manifest, and selection records give no such bounds. We calculated sufficient center-movement margins for 78 known two-crossing intervals and one known 10 µm case, using fixed straight-segment coordinates. Margins ranged from approximately 0.00000176300517 to 0.0150251624381 µm (median approximately 0.000385159997827 µm); 50 of 78 were below 0.001 µm. All 158 constructed movements beyond a margin removed the relevant double crossing. The 10 µm case had a margin of approximately 0.002296606109251317 µm; a constructed movement of approximately 0.002319572170343830 µm changed whole-cell counts from 8 endpoint and 10 internal crossings to 8 and 8. All 1,264 specified half-margin checks and 12 artificial method checks passed, and binary64 validation agreed within the recorded tolerance. These are geometric sensitivity results, not measured tissue error, population error rates, or evidence about cell function. The 12 Allen SWC files are identified by source URLs and hashes but omitted from the SCI ZIP under their recorded distribution terms.

Sholl analysis · neuron morphology · center error · sensitivity analysis · computational geometry · mouse neuron traces