Endpoint counts are too small at some radii in all 12 tested mouse neuron traces.

In a fixed, nonrandom sample of 12 mouse neuron traces from the Allen Cell Types Database, every trace had at least one radius interval where an endpoint method missed two internal sphere crossings. Decimal calculations identified 78 segments with such open intervals. NeuroM 4.0.6 returned 8 instead of 10 crossings at 10 µm for specimen 314642645 on matched loaded dendrite sections. New code reproduced all 4,968 prior cell-radius counts. The 1 µm and 10 µm grids each differed on one pair, at the same radius and specimen. A common grid phase intersected at least one interval in 22.4017% of phases for a 1 µm grid and 2.35949% for a 10 µm grid in these stored files; these are not probabilities for living neurons. The result concerns stored straight centerlines. Small coordinate or center changes can alter the narrow intervals, and the study does not establish effects on classification, physiology, tissue accuracy, or population prevalence. The source SWCs are identified by recorded URLs and hashes; an immutable dataset release is not established.

Sholl analysis · neuron morphology · sphere intersections · endpoint counting · computational geometry · NeuroM · Allen Cell Types Database · reproducibility